基于RNA-seq技术对乌头属铁棒锤中自交不亲和S基因的挖掘与分析  

Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N.Busch based on RNA-seq

作　　者：李雪丽 耿甜甜 汪颖 钱婷 张玉洁 赵凡 孙坤[1] 张辉[1] Li Xue-Li;Geng Tian-Tian;Wang Ying;Qian Ting;Zhang Yu-Jie;Zhao Fan;Sun Kun;Zhang Hui(College of Life Science, Northwest Normal University, Lanzhou 730070, China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《植物科学学报》2021年第2期172-182,共11页Plant Science Journal

基　　金：中国科学科院遗传发育研究所染色体工程国家重点实验室开放基金项目(PCCE-KF-2019-06);国家自然科学基金项目(31660060,31060033)。

摘　　要：以毛茛科乌头属铁棒锤(Aconitum pendulum N.Busch)2个品系‘蓝花铁棒锤’(‘WSYB1’)和‘黄花铁棒锤’(‘WSYY1’)为材料,对其进行转录组测序(RNA-seq),采用生物信息学方法鉴定其中可能存在的花柱S基因(self-incompatibility gene)和花粉S基因,并对它们的序列特征进行分析。结果显示,转录组中共鉴定出2个在雌蕊中特异或高表达的花柱S基因(ApSRNase)和2个在雄蕊中特异表达的花粉S基因(ApSLF)。与耧斗菜(Aquilegia coerulea James)相似,铁棒锤中也存在S-RNase(S locus ribonucleases)和SLF(S locus F-box)控制的S-RNase类的自交不亲和系统,而不存在sS(stigma S-determinant)和pS(pollen S-determinant)控制的罂粟科类型的自交不亲和系统。Two Aconitum pendulum N.Busch cultivars(‘WSYB1’and‘WSYY1’)were used as materials for transcriptome sequencing(RNA-seq)using bioinformatics to identify possible style and pollen S genes and analyze their sequence characteristics.Results identified two style S genes(ApSRNase)specifically or highly expressed in the style and two pollen S genes(ApSLF)specifically expressed in pollen.Similar to the sequenced plant Aquilegia coerulea James,this study showed that there was a S-RNase-based self-incompatibility system controlled by S-RNase and SLF in A.pendulum,but no Papaveraceae-type self-incompatibility system controlled by sS and pS.

关 键 词：铁棒锤 RNA-SEQ 自交不亲和性 S-RNASE基因 SLF基因 

分 类 号：Q943.2[生物学—植物学]