Patient-specific induced pluripotent stem cells as“disease-in-adish”models for inherited cardiomyopathies and channelopathies–15 years of research  

作　　者：Miruna Mihaela Micheu Ana-Maria Rosca 

机构地区：[1]Department of Cardiology,Clinical Emergency Hospital of Bucharest,Bucharest 014452,Romania [2]Cell and Tissue Engineering Laboratory,Institute of Cellular Biology and Pathology"Nicolae Simionescu",Bucharest 050568,Romania

出　　处：《World Journal of Stem Cells》2021年第4期281-303,共23页世界干细胞杂志（英文版）（电子版）

摘　　要：Among inherited cardiac conditions,a special place is kept by cardiomyopathies(CMPs)and channelopathies(CNPs),which pose a substantial healthcare burden due to the complexity of the therapeutic management and cause early mortality.Like other inherited cardiac conditions,genetic CMPs and CNPs exhibit incomplete penetrance and variable expressivity even within carriers of the same pathogenic deoxyribonucleic acid variant,challenging our understanding of the underlying pathogenic mechanisms.Until recently,the lack of accurate physiological preclinical models hindered the investigation of fundamental cellular and molecular mechanisms.The advent of induced pluripotent stem cell(iPSC)technology,along with advances in gene editing,offered unprecedented opportunities to explore hereditary CMPs and CNPs.Hallmark features of iPSCs include the ability to differentiate into unlimited numbers of cells from any of the three germ layers,genetic identity with the subject from whom they were derived,and ease of gene editing,all of which were used to generate“disease-in-a-dish”models of monogenic cardiac conditions.Functionally,iPSC-derived cardiomyocytes that faithfully recapitulate the patient-specific phenotype,allowed the study of disease mechanisms in an individual-/allele-specific manner,as well as the customization of therapeutic regimen.This review provides a synopsis of the most important iPSC-based models of CMPs and CNPs and the potential use for modeling disease mechanisms,personalized therapy and deoxyribonucleic acid variant functional annotation.

关 键 词：Induced pluripotent stem cells CARDIOMYOPATHY CHANNELOPATHY Genes Mutation Deoxyribonucleic acid variants 

分 类 号：R541[医药卫生—心血管疾病]