绝经后骨质疏松症差异表达lncRNAs及其靶基因抑瘤素M在外周血的表达研究  

Differential expression of lncRNAs and its target gene oncostatin M in peripheral blood in postmenopausal osteoporosis

作　　者：李生强 谢冰颖 陈娟 谢丽华 叶云金 黄景文 葛继荣 LI Shengqiang;XIE Bingying;CHEN Juan;XIE Lihua;YE Yunjin;HUANG Jingwen;GE Jirong(Key Research Section of Osteoporosis Syndrome Genomics, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, China)

机构地区：[1]福建省中医药科学院骨质疏松证候基因组学研究室,福建福州350003

出　　处：《中国骨质疏松杂志》2021年第3期359-363,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(81774350);福建省自然科学基金项目(2019J01339);福建省科技厅省属公益类科研院所基本科研专项(2019R1003-1)。

摘　　要：目的探讨绝经后骨质疏松症差异表达lncRNAs及其靶基因抑瘤素M(oncostatin M,OSM)在绝经后骨质疏松症外周血的表达及其意义。方法在前期lncRNA-mRNA芯片检测绝经后骨质疏松症差异lncRNAs并进行共表达分析的基础上,利用blat软件分析lncRNA TTC28-AS1、linc-IRF2BP2-1、linc-FAM126B-1靶基因;RNAfold预测其二级结构;随机选择绝经后妇女受试者,检测骨密度,正常骨密度妇女25例为对照组,骨质疏松症患者25例为骨质疏松症组;定量PCR检测两组人群外周血lncRNA TTC28-AS1、linc-IRF2BP2-1、linc-FAM126B-1及抑瘤素M的表达。结果blat软件预测lncRNA TTC28-AS1、linc-IRF2BP2-1、linc-FAM126B-1的靶基因为OSM,相关系数分别为0.8506(P=0.0037)、0.9314(P=0.0003)及0.9222(P=0.0004);RNAfold预测3个lncRNAs均具有多个茎环、多分枝内部环结构;lncRNA TTC28-AS1、linc-IRF2BP2-1、linc-FAM126B-1及其靶基因抑瘤素M在骨质疏松症组中表达水平均显著低于对照组(P值均小于0.05)。结论lncRNA TTC28-AS1、linc-IRF2BP2-1、linc-FAM126B-1及其靶基因抑瘤素M表达下调可能与绝经后骨质疏松症存在相关性。Objective Our aim is to investigate the expression and significance of differential expressed long-chain non-coding RNAs and oncostatin M(OSM)in postmenopausal osteoporosis.Methods Based on the detection of lncRNAs with lncRNA-mRNA chip and co-expression analysis,the target genes lncRNA TTC28-AS1,linc-IRF2BP2-1,and linc-FAM126B-1 were analyzed with a blat software.The secondary structure of lncRNAs was predicted with an online software RNAfold.The subjects were randomly selected from the postmenopausal women,and their BMD was measured.Twenty-five women with normal BMD were included in the control group.Twenty-five patients with osteoporosis were included in the osteoporosis group.The expression of oncostatin M and lncRNA TTC28-AS1,linc-IRF2BP2-1,and linc-FAM126B-1 in the two groups were detected with quantitative PCR.Results Blat software predicted that the target of lncRNA TTC28-AS1,linc-IRF2bp2-1,and linc-FAM126B-1 was OSM,and the correlation coefficients were 0.8506(P=0.0037),0.9314(P=0.0003),and 0.9222(P=0.0004)respectively.RNAfold predicted that all the 3 lncRNAs had multiple stem rings and multi-branched internal ring structures.The expression levels of OSM,lncRNA TTC28-AS1,linc-IRF2bp2-1,and linc-FAM126B-1 in postmenopausal osteoporosis group were significantly lower than those in the control group(P<0.05).Conclusion The down-regulation of lncRNA TTC28-AS1,linc-IRF2BP2-1,linc-FAM126B-1,and their target gene oncostatin M may be related to postmenopausal osteoporosis.

关 键 词：长链非编码RNA 抑瘤素M 绝经后骨质疏松症 RNA二级结构 lncRNA TTC28-AS1 linc-IRF2BP2-1 linc-FAM126B-1 

分 类 号：R681[医药卫生—骨科学]