新型表面麻醉剂在新西兰兔口腔黏膜麻醉中的作用机制研究  

Study on the mechanism of new type of topical anesthetic in oral mucosal anesthesia of New Zealand rabbits

作　　者：曾东 牛娇 李长义[3] 常津 牛光良 ZENG Dong;NIU Jiao;LI Changyi;CHANG Jin;NIU Guangliang(Department of Dentistry,Bejing Hospital of Integrated Traditional Chinese and Western Medicine,Beijing100039,China;Department of Dentistry,the Second Hospital of Shanxi Medical University,Shanxi Province,Taiyuan030001,China;Department of Hospital Office,Tianjin Medical University,Tianjin300070,China;Institute of Nanomedicine Tianjin University School of Life Sciences,Tianjin300072,China)

机构地区：[1]北京市中西医结合医院口腔科,北京100039 [2]山西医科大学第二医院口腔科,山西太原030001 [3]天津医科大学口腔医院院办,天津300070 [4]天津大学生命科学学院纳米医学研究所,天津300072

出　　处：《中国医药导报》2021年第12期28-32,共5页China Medical Herald

基　　金：首都卫生发展科研专项项目(首发2014-4-7042)。

摘　　要：目的探讨新研制的口腔表面麻醉剂-载盐酸利多卡因葡聚糖基纳米高分子脂质体(LID-LLD-PLVs)的口腔黏膜表面麻醉作用机制。方法将24只8月龄新西兰兔按体重分层随机分组法分为4组,每组6只,为空白对照组(不给药)、下颌前牙唇侧牙龈表面涂抹LID-LLD-PLVs 0.5 mL,按作用时间分为LID-LLD-PLVs 30 min、60 min和90 min组。然后于各组下前牙黏膜注射10%福尔马林溶液25μL后60 min,取给药部位牙龈、同侧三叉神经节和延髓。应用实时荧光定量PCR方法对牙龈、三叉神经节和延髓的P物质(SP)、降钙素基因相关肽(CGRP)和c-fos进行mRNA定量分析。结果LID-LLD-PLVs 30 min、60 min和90 min组的牙龈、三叉神经节和延髓的SP、CGRP和c-fos mRNA定量均低于对照组,差异均有统计学意义(均P<0.05)。结论LID-LLD-PLVs的作用机制可能与抑制了牙龈、三叉神经节和延髓中SP、CGRP和c-fos的表达水平有关。Objective To investigate the mechanism of oral mucosal surface anesthesia with new type of oral topical anesthetic which as Lidocaine hydrochloride lysine-linoleic acid modified dextran polymeric lipid vesicles(LID-LLD-PLVs).Methods Twenty-four New Zealand rabbits aged eight months were randomly divided into four groups randomly according to body weight layer according to the random number table method,and six in each group,which were blank control group(no administration),0.5 mL LID-LLD-PLVs were applied on the gingival surface of mandibular anterior teeth,then the group was divided into LD-LD-PLVS 30 min,60 min and 90 min groups according to the action time.Then the anterior mucosa of each group was injected with 25μL of 10%formalin solution for 60 min,and the gingiva,ipsilateral trigeminal ganglion and medulla oblongata were extracted.Real-time PCR was used to quantitatively analyze the mRNAs of substance P(SP),calcitonin gene related peptide(CGRP)and c-fos in gingival,trigeminal ganglion and medulla oblongata.Results The mRNA quantitative of SP,CGRP and c-fos in the gingival,trigeminal ganglion and medulla oblongata of LID-LLD-PLVs 30 min,60 min and 90 min groups were all lower than that of the control group,and the differences were statistically significant(all P<0.05).Conclusion The mechanism of LID-LLD-PLVs may be related to the inhibition of the expression levels of SP,CGRP and c-fos in gingival,trigeminal ganglion and medulla oblongata.

关 键 词：表面麻醉剂-载盐酸利多卡因葡聚糖基纳米高分子脂质体 纳米脂质体 利多卡因 口腔表面麻醉 P物质 降钙素基因相关肽 C-FOS 

分 类 号：R781[医药卫生—口腔医学]