犬瘟热病毒YD株的分离鉴定及其H基因序列分析  

Isolation and identification of caine distemper virus strain YD and sequence analysis of its H gene

作　　者：张建普 马丽利 周利锋 ZHANG Jianpu;MA Lili;ZHOU Lifeng(Yanshi Animal Health Supervision Institute,Yanshi 471900,China;Shanghai Qisheng Biological Technology Co.,Ltd.,Shanghai 201203,China)

机构地区：[1]河南省偃师市动物卫生监督所,河南偃师471900 [2]上海启盛生物科技有限公司,上海201203

出　　处：《畜牧与兽医》2021年第4期69-75,共7页Animal Husbandry & Veterinary Medicine

摘　　要：从疑似犬瘟热(CD)病犬的脏器中分离到1株病毒,经间接免疫荧光试验、RT-PCR鉴定、红细胞凝集试验和对不同动物致病性试验,证实该病毒为犬瘟热病毒(CDV)强毒株,命名为CDV YD株。对H基因序列的测定和分析表明,YD株H基因与国内强毒株更接近,核苷酸同源性为98.4%~99.7%,氨基酸同源性为97.8%~99.7%,而与疫苗株核苷酸同源性较远为91.2%~91.5%,氨基酸同源性为90.3%~91.2%。推导的H蛋白氨基酸序列中有9个潜在的N连接糖基化位点(N-X-S/T),可能与病毒体外复制和中和抗体有关,另外有12个保守的半胱氨酸(Cys)残基,对H蛋白二级结构起重要作用;根据H基因核苷酸序列绘制的进化树表明,CDV YD株属于国内流行基因型:Asia-1型。该研究为了解当前中国CDV流行变异情况和犬瘟热疫苗的研发提供了数据。A strain of virus was isolated from the organs of a dog suspected with canine distemper(CD).The virulent virus,designated as YD,was identified as canine distemper virus(CDV)by indirect immunofluorescence assay,RT-PCR,haemagglutination and pathogenesis in different animals.Analysis of the H gene derived from the virulent strain revealed that the YD strain had 98.4%-99.7%nucleotide sequence homology and 97.8%-99.7% homology in putative amino acid sequences,especially sharing a high degree of homology with the Chinese virulent strain;but,compared with the existing vaccine strains,the homologies of YD strain were 91.2%-91.5% in the nucleotide sequence and 90.3%-91.2% in the amino acid sequence.Nine potential sites for N-linked glycosylation which might be related to virus neutralization by antibodies and replication in vitro were identified in the H protein of the YD strain.In addition,twelve conservative cysteine residues known to be important for the secondary structure of the H protein were also found in its predicted amino acid sequences.Phylogenetic analysis of the N and H genes showed that the YD strain belonged with the Asia-1 group,the predominant genotype in China.This study offered a basis for a better understanding of genetic variation of CDV in China and for development of CD vaccine.

关 键 词：犬瘟热病毒 分离 鉴定 H基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]