SPE、SIL及IT技术对多发性骨髓瘤的临床诊断价值研究  

Study on the clinical diagnostic value of SPE,SIL and IT technology in multiple myeloma

作　　者：徐传华 杨伟 余晓辉 袁世梅 刘琼 程颖[2] 罗淋丹 XU Chuanhua;YANG Wei;YU Xiaohui;YUAN Shimei;LIU Qiong;CHENG Ying;LUO Lindan(Department of Laboratory Medicine,People′s Hospital of Dianjiang County,Chongqing,Chongqing 408300,China;Chongqing Blood Center,Chongqing 400015,China)

机构地区：[1]重庆市垫江县人民医院检验科,重庆408300 [2]重庆市血液中心,重庆400015

出　　处：《现代医药卫生》2021年第9期1462-1464,共3页Journal of Modern Medicine & Health

基　　金：重庆市垫江县科学技术局科研项目(djkjzdxm2019jsyfysfyy001)。

摘　　要：目的探讨血清蛋白电泳(SPE)、血清免疫球蛋白定量(SIL)和毛细管电泳免疫分型(IT)技术在多发性骨髓瘤(MM)诊断和分型中的临床应用价值。方法收集2019年1月至2020年5月重庆市垫江县人民医院临床确诊和疑似MM的137例患者样本,分别采用SPE、SIL及IT技术进行检测。结果137例患者样本中,SIL检出阳性样本83例,阳性检出率为60.58%;SPE检测发现含有单克隆免疫球蛋白(M蛋白)样本113例,阳性检出率为82.48%;IT检出阳性样本131例,阳性检出率为95.62%。3种方法阳性检出率比较,差异有统计学意义(P<0.05)。结论SPE和SIL技术可协助临床初筛患者是否含有M蛋白。IT技术可用于对M蛋白的鉴定和分型,并对临床MM的诊断分型及疗效预后判断有重要的指导意义。Objective To explore the clinical value of serum protein electrophoresis(SPE),serum immunoglobulin levels(SIL)and capillary electrophoresis immunotyping(IT)techniques in the diagnosis and typing of multiple myeloma(MM).Methods From January 2019 to May 2020,samples of 137 cases patients with clinically confirmed and suspected MM were collected in the hospital,and SPE,SIL and IT technologies were used for testing.Results Among the Samples of 137 cases patients,83 cases samples were positive for SIL,and the positive detection rate was 60.58%;113 cases were found to contain monoclonal immunoglobulin(M protein)samples by SPE,and the positive detection rate was 82.48%;131 cases positive samples were detected by IT,and the positive detection rate was 95.62%.The positive detection rates of the three methods were compared,and the difference was statistically significant(P<0.05).Conclusion SPE and SIL technology can assist in the preliminary clinical screening of patients for M protein.IT technology can be used for the identification and classification of M protein,and has important guiding significance for the clinical diagnosis and prognosis of MM.

关 键 词：多发性骨髓瘤 血清蛋白电泳 血清免疫球蛋白定量 毛细管电泳免疫分型 M蛋白 免疫分型 

分 类 号：R-331[医药卫生]