芦荟苦素和芦荟凝胶对新月弯孢的抑菌活性  

Inhibitory activity of aloesin and aloe gel against Curvularia lunata

作　　者：张国辉 李荣玉[1,2] 吴小毛 李明[1,2] ZHANG Guohui;LI Rongyu;WU Xiaomao;LI Ming(Institute of Crop Protection,Guizhou University,Guiyang 550025,China;The Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region,Guiyang 550025,China;School of Life and Health Science,Kaili University,Kaili 556000,Guizhou Province,China)

机构地区：[1]贵州大学作物保护研究所,贵阳550025 [2]贵州省山地农业病虫害重点实验室,贵阳550025 [3]凯里学院大健康学院,贵州凯里556000

出　　处：《农药学学报》2021年第2期316-322,共7页Chinese Journal of Pesticide Science

基　　金：supported by National Key R&D Program of China(No.2016YFD0200500);the national natural sci-ence foundation of China(No.31701816,31460480);agricultural research projects of the science and technology department of Guizhou province(No.[2019]3001);the science and technique foundation of Guizhou province(No.[2017]1083)。

摘　　要：通过研究芦荟苦素和芦荟凝胶对新月弯孢Curvularia lunata的抑菌效果,以期寻找天然活性物质用于水稻新月弯孢叶斑病的防治。滤纸法显示:300 mg/mL的芦荟苦素处理28 h后对新月弯孢可产生明显的抑制带(3.37 mm);在孢子液(3×10^(4)cfu/mL)平板上可产生直径为51.27 mm的抑菌圈,且抑制效应可达6 d。用1、2、4、8、16、32、64 mg/mL系列质量浓度的芦荟凝胶处理新月弯孢5 d时的菌丝抑制率范围为9.25%~56.21%,抑菌率随芦荟凝胶质量浓度的增加而升高。此外,从处理后2 d到5 d,64 mg/mL芦荟凝胶的抑制率增加了21.87%。显微镜检表明,160 mg/mL芦荟苦素处理的C.lunata菌丝生长弱于对照,且无附着胞产生。经芦荟凝胶处理后,分生孢子芽管的生长速度显著低于对照,其生长抑制率可达75.39%-96.58%。本研究首次报道芦荟苦素能有效抑制C.lunata的菌丝生长和分生孢子萌发,而芦荟凝胶能有效抑制C.lunata的菌丝和芽管生长。In order to find natural active substance alternative to the chemical fungicides currently used against the devastating Curvularia leaf spot of rice,the bioactivity of aloesin and aloe gel against Curvularia lunata was determined.The filter paper method showed that 300 mg/mL aloesin led to an obvious inhibitory band(3.37 mm)at 28 h,and a inhibitory zone(Φ=51.27 mm)against spores(3×10_(4) cfu/mL),and these inhibitory effects remained until 6 d.The mycelial inhibition rate by aloe gel at 1,2,4,8,16,32 and 64 mg/mL ranged from 9.25%to 56.21%at 5 d,and it increased with increased concentration.Additionally,the inhibition rate of 64 mg/mL aloe gel increased by 21.87%from 2 to 5 d.The mycelial growth of C.lunata treated with 160 mg/mL aloesin was weaker than control,and no appressorium produced.The germ tube of C.lunata treated with aloe gel was much slower than control,and the inhibition rate was 75.39%-96.58%.This study was first reported that aloesin effectively inhibited mycelia growth and conidia germination of C.lunata,and aloe gel effectively inhibited mycelia growth of C.lunata.

关 键 词：芦荟苦素 芦荟凝胶 新月弯孢(Curvularia lunata) 抑菌活性 

分 类 号：TQ458.2[化学工程—农药化工] S435.111[农业科学—农业昆虫与害虫防治]