卵巢癌组织中miR-410、CCNB1的表达及临床意义  

Expression and clinical significance of miR-410 and CCNB1 in ovarian cancer tissues

作　　者：赵爽 孙静莉 ZHAO Shuang;SUN Jingli(Department of Obstetrics and Gynecology,General Hospital of the Northern Theater of the Chinese People’s Liberation Army,Shenyang Province,Liaoning110003,China)

机构地区：[1]中国人民解放军北部战区总医院妇产科,辽宁沈阳110003

出　　处：《中国医药导报》2021年第11期105-108,共4页China Medical Herald

基　　金：辽宁省卫生计生委科研课题(201703106)。

摘　　要：目的探讨卵巢癌组织中微小RNA-410(miR-410)与细胞周期蛋白B1(CCNB1)的表达水平,并分析其与临床病理资料之间的关系。方法应用荧光实时定量PCR技术(qRT-PCR)检测中国人民解放军北部战区总医院(2017年1月—2020年2月)83例卵巢癌组织和癌旁组织中miR-410与CCNB1 mRNA的相对表达量,分析其与患者临床病理资料的关系。结果卵巢癌组织中miR-410的相对表达量低于癌旁组织,CCNB1 mRNA的相对表达量高于癌旁组织(P<0.05)。卵巢癌组织中miR-410在肿瘤FIGOⅢ~Ⅳ期、肿瘤浸润深度T3+T4、细胞分化低~中分化及有淋巴结转移的相对表达量低于肿瘤FIGOⅠ~Ⅱ期、肿瘤浸润深度T1+T2、细胞分化高分化及无淋巴结转移的患者,CCNB1的相对表达量在肿瘤FIGOⅢ~Ⅳ期、肿瘤浸润深度T3+T4、细胞分化低~中分化及有淋巴结转移于高于肿瘤FIGOⅠ~Ⅱ期、肿瘤浸润深度T1+T2、细胞分化高分化及无淋巴结转移(P<0.05)。相关分析结果显示,卵巢癌组织中miR-410与CCNB1的表达呈负相关(r=-0.605,P<0.05)。结论卵巢癌组织中miR-410表达水平低于癌旁组织,CCNB1 mRNA表达水平高于癌旁组织,与肿瘤FIGO分期、肿瘤浸润深度、细胞分化程度及淋巴转移有紧密联系,有望成为卵巢癌诊断、治疗以及评估预后的新的生物学指标。Objective To investigate the expression levels of microRNA-410(miR-410)and cyclin B1(CCNB1)in ovarian cancer,and to analyze their relationship with clinical pathological data.Methods Quantitative real-time PCR(qRT-PCR)was used to detect the relative expression of miR-410 and CCNB1 mRNA in 83 cases of ovarian cancer and adjacent tissues in General Hospital of the Northern Theater of the Chinese People’s Liberation Army(from January 2017 to February 2020),the relationship between the two indicators and clinical pathological data was analyzed.Results The relative expression of miR-410 in ovarian cancer was lower than in adjacent tissues,and the relative expression of CCNB1 mRNA was higher than in adjacent tissues(P<0.05).The relative expression of miR-410 in ovarian cancer tissues in FIGO stageⅢ-Ⅳ,tumor invasion depth T3+T4,low-medium cell differentiation,and lymph node metastasis was lower than those of tumor FIGO stageⅠ-Ⅱ,tumor invasion depth T1+T2,patients with high cell differentiation and had no lymph node metastasis,and the relative expression of CCNB1 in the tumor FIGO stageⅢ-Ⅳ,the tumor infiltration depth was T3+T4,the cell differentiation was low to medium,and the lymph node metastasis was higher than those of the tumor FIGO stageⅠ-Ⅱ,the depth of tumor invasion was T1+T2,and the cells were highly differentiated and had no lymph node metastasis(P<0.05).Correlation analysis showed that there was a negative correlation between miR-410 and CCNB1 expression in ovarian cancer(r=-0.605,P<0.05).Conclusion The expression level of miR-410 in ovarian cancer is lower than that in adjacent tissues,and the expression level of CCNB1 mRNA is higher than in adjacent tissues,which is closely related to the tumor FIGO stage,tumor invasion depth,cell differentiation degree and lymphatic metastasis.It is expected to become an biomarkers for ovarian cancer diagnosis,treatment and prognosis.

关 键 词：卵巢癌 miR-410 细胞周期蛋白B1 淋巴转移 

分 类 号：R737.33[医药卫生—肿瘤]