多指标综合评分法优选黄芪三七合剂提取工艺  

Optimization of extraction process of Astragalus and Radix Notoginseng by multi index comprehensive score method

作　　者：兰杨 张仕瑾 王娅俐 郭廷东[1] 唐志立[1] 谢席胜[2] LAN Yang;ZHANG Shijin;WANG Yali;GUO Tingdong;TANG Zhili;XIE Xisheng(Nanchong Key Laboratory of Individualized Drug Therapy,Department of Pharmacy,the Second Clinical Medical College of North Sichuan Medical CollegeNanchong Central Hospital,Sichuan Province,Nanchong637000,China;Department of Nephrology,the Second Clinical Medical College of North Sichuan Medical CollegeNanchong Central Hospital,Sichuan Province,Nanchong637000,China)

机构地区：[1]川北医学院第二临床医学院四川省南充市中心医院药学部个体化药物治疗南充市重点实验室,四川南充637000 [2]川北医学院第二临床医学院四川省南充市中心医院肾内科,四川南充637000

出　　处：《中国医药导报》2021年第10期23-25,30,共4页China Medical Herald

基　　金：四川省科技计划项目(21ZDYF2053);四川省中医药管理局中医药科研专项(2018JC037、2018YY007);四川省教育厅科研计划项目(18ZB0223);四川省南充市首批重点实验室建设项目(NCKL201711);四川省南充市市校合作科研专项(NSMC20170109、18SXHZ0453、19SXHZ0346);川北医学院科研发展计划项目(CBY18-A-YB32)。

摘　　要：目的以多指标综合评分法结合正交设计优选黄芪三七合剂提取工艺。方法以处方中有效成分毛蕊异黄酮葡萄糖苷、阿魏酸、人参皂苷Rg1含量以及浸膏得率的综合评分为评价指标,采用正交试验考察加水倍数、提取时间、提取次数对综合评分的影响。结果优选的提取工艺为加10倍量水,提取4次,每次30 min。在此条件下,得到毛蕊异黄酮葡萄糖苷、阿魏酸、人参皂苷Rg1平均含量均值分别为0.61、1.51、26.23 mg/g,浸膏得率为41.02%,RSD分别为0.94%、1.38%、1.20%、0.71%(n=3)。结论优选的工艺稳定可行,可作为该制剂的提取工艺。Objective To optimize the extraction process of Astragalus and Radix Notoginseng by multi index comprehensive score method combined with orthogonal design.Methods The contents of isoflavone glucoside,ferulic acid,ginsenoside Rg1 in the prescription and the comprehensive score of extract yield were used as evaluation indexes.The effects of water multiple,extraction time and extraction times on the comprehensive score were investigated by orthogonal test.Results The optimal extraction process was to add ten times of water,extract four times,30 min each time.Under these conditions,the average content of isoflavone glucoside,ferulic acid,ginsenoside Rg1 were 0.61,1.51,26.23 mg/g respectively,and the and extract yield was 41.02%,and the RSD was 0.94%,1.38%,1.20%,0.71%,respectively(n=3).Conclusion The optimized process is stable and feasible,which can be used as the extraction process of this preparation.

关 键 词：黄芪三七合剂 多指标综合评分 正交设计 毛蕊异黄酮葡萄糖苷 阿魏酸 人参皂苷RG1 浸膏得率 

分 类 号：R284.2[医药卫生—中药学]