靶向阻断CXCR4对上皮性卵巢癌细胞CD44和CD133表达的影响  

Effect of targeted blockade CXCR4 on CD44 and CD133 expression in epithelial ovarian cancer cell

作　　者：陈永香 杨梦月 彭仁国 曹言言 訾聃 CHEN Yongxiang;YANG Mengyue;PENG Renguo;CAO Yanyan;ZI Dan(College of Clinical Medical,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Obstetrics and Gynecology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Center for Tissue Engineering and Stem Cell Experiment,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学临床医学院,贵州贵阳550025 [2]贵州医科大学附属医院妇产科,贵州贵阳550004 [3]贵州医科大学组织工程与干细胞实验中心,贵州贵阳550025

出　　处：《贵州医科大学学报》2021年第4期410-414,446,共6页Journal of Guizhou Medical University

基　　金：贵州省教育厅科技拔尖人才支持项目[黔教合KY字(2016)072];贵阳市科技计划攻关项目[筑科合同(2018)1-91];贵州省贵阳市科技局国基培育项目(筑科合同GY2015-40)。

摘　　要：目的探讨趋化因子受体4(CXCR4)靶向阻断剂普乐沙福(AMD3100)处理上皮性卵巢癌细胞(OVCAR-3)后对其干细胞特征相关蛋白分化抗原簇44(CD44)和分化抗原簇133(CD133)的影响。方法培养至对数期生长的OVCAR-3细胞,分为对照组(0 mg/L AMD3100)、低浓度组(10 mg/L AMD3100)及高浓度组(100 mg/L AMD3100),采用Western blot检测各组OVCAR-3细胞中CXCR4、CD44及CD133蛋白的表达,采用细胞悬浮成球实验检测第2天和第7天时各组OVCAR-3细胞悬浮球的成球率。结果低浓度组和高浓度组OVCAR-3细胞CXCR4、CD133及CD44蛋白表达分别低于对照组,且高浓度组也低于低浓度组,差异均有统计学意义(P<0.05);培养至第7天时,对照组OVCAR-3细胞悬浮成球率分别低于低浓度组和高浓度组,且低浓度组也低于高浓度组,差异均有统计学意义(P<0.05或P<0.01)。结论靶向阻断CXCR4可降低OVCAR-3细胞中CD44和CD133的表达。Objective To investigate the effect of chemokine receptor 4(CXCR4)targeting blocker Plexaf(AMD3100)on the differentiation antigen cluster 44(CD44)and differentiation antigen cluster 133(CD133)of epithelial ovarian cancer cells(epithelial ovarian cancer cells)after treatment.Methods OVCAR-3 cells grown to logarithmic phase were divided into control group(0 mg/L AMD3100),low concentration group(10 mg/L AMD3100)and high concentration group(100 mg/L AMD3100).The CXCR4,CD44,and CD133 proteins in OVCAR-3 cells of each group were detected by using the Western blot.Cell suspension ball formation test was used to detect the ball formation rate of OVCAR-3 cells on day 2 nd and 7 th.Results The CXCR4,CD133,and CD44 protein expression of OVCAR-3 cells in low concentration group and high concentration group were lower than those in control group,and the high concentration group was lower than that in low concentration group.The difference was statistically significant(P<0.05).At the 7 th day,the suspension rate of OVCAR-3 cells in the control group was lower than that in the low concentration group and the high concentration group,respectively,and the low concentration group was also lower than the high concentration group.The difference was statistically significant(P<0.05 or P<0.01).Conclusion Targeting blocking CXCR4 reduces CD44 and CD133 expression in OVCAR-3 cells.

关 键 词：卵巢肿瘤 趋化因子受体4 靶向阻断 普乐沙福 干细胞特征 

分 类 号：R737.31[医药卫生—肿瘤]