腹腔内注射骨髓间充质干细胞对急性胰腺炎小鼠相关肺损伤的影响及相关机制研究  

Effects of intraperitoneal injection of bone marrow mesenchymal stem cells on lung injury associated with acute pancreatitis in mice and its mechanism

作　　者：马博[1] 周军[2] 李建刚[1] 王俊 李亮[1] MA Bo;ZHOU Jun;LI Jian-gang(Department of General Surgery,Second Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830063,China;Department of Gastrointestinal Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou Guangdong 510120,China)

机构地区：[1]新疆医科大学第二附属医院普外科,新疆乌鲁木齐830063 [2]中山大学孙逸仙纪念医院胃肠外科,广东广州510120

出　　处：《临床和实验医学杂志》2021年第7期673-676,共4页Journal of Clinical and Experimental Medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(编号:2016D01C202)。

摘　　要：目的研究腹腔内注射骨髓间充质干细胞(BMSC)对雨蛙素诱导的急性胰腺炎(AP)小鼠肺损伤的影响,并进一步探讨其可能的机制。方法选取Balb/c小鼠作为研究对象,分为Sham组(注射0.9%氯化钠溶液),AP组(腹腔注射雨蛙素诱导AP模型)和AP+BMSC组(经BMSC治疗的AP模型),每组各8只。分别在AP诱导成功后12、24、48 h,收集小鼠血液并处死小鼠,测定不同组小鼠肺组织湿/干重比值,并用苏木精-伊红染色法评估小鼠肺组织病理变化,并检测3组小鼠肺组织髓过氧化物酶(MPO)活性和血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、淀粉酶(AMY)以及丙二醛(MDA)含量。结果与Sham组相比,AP组和AP+BMSC组小鼠在诱导建立AP模型后12、24、48 h肺组织湿/干重比值、血清淀粉酶、肺组织MPO以及血清TNF-α、IL-6、IL-10和丙二醛均显著升高,差异均有统计学意义(P<0.05);与AP组小鼠相比,AP+BMSC组小鼠在诱导建立AP模型后12、24、48 h肺组织湿/干重比值、血清淀粉酶、肺组织MPO以及血清TNF-α、IL-6、IL-10和丙二醛均显著降低,血清IL-10含量显著升高,差异均有统计学意义(P<0.05)。结论腹腔内注射骨髓间充质干细胞可显著减弱AP引起的肺损伤,其作用机制可能与改善AP小鼠氧化应激及炎症反应水平有关。Objective To study the effect of intraperitoneal injection of bone marrow mesenchymal stem cell(BMSC)on the lung injury of acute pancreatitis(AP)mice induced by caerulein,and further explore its possible mechanism.Methods Balb/c mice were selected as the research objects,and they were divided into Sham group(injection of physiological saline),AP group(induction of AP model by intraperitoneal injection of caerulein)and AP+BMSC group(AP model treated with BMSC),each group 8 mice.they were successfully induced in AP respectively After 12,24 and 48 h,blood was collected from the mice and the mice were sacrificed.The wet/dry weight ratios of the lung tissues of different groups of mice were measured,and the hematoxylin-eosin staining method was used to evaluate the pathological changes of the lung tissues of the mice.The activity of myeloperoxidase(MPO)and levels of serum tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10),amylase(AMY)and malondialdehyde(MDA)were detected in each group.Results Compared with the Sham group,mice in the AP group and AP+BMSC group at 12,24 and 48 h after treatment,lung tissue wet/dry weight ratio,serum amylase,lung tissue MPO,and serum TNF-α,IL-6,IL-10 and malondialdehyde were all significantly increased,the differences were statistically significant(P<0.05).Compared with the AP group mice,the AP+BMSC group mice at 12,24 and 48 h after treatment,lung tissue wet/dry weight ratio,serum amylase,lung tissue MPO and serum TNF-α,IL-6,IL-10 and malondialdehyde were all significantly reduced,whlie serum IL-10 content was significantly increased,the differences were statistically significant(P<0.05).Conclusion Intraperitoneal injection of bone marrow mesenchymal stem cells can significantly reduce lung injury caused by acute pancreatitis,and its mechanism of action may be related to the improvement of oxidative stress and inflammation in pancreatitis mice.

关 键 词：小鼠 急性胰腺炎 肺损伤 骨髓间充质干细胞 炎症 氧化应激 

分 类 号：R576[医药卫生—消化系统]