普通小麦溶剂保持力全基因组关联分析  

Genome-wide association analysis on solvent retention capacity in common wheats

作　　者：刘巧 方正武[1] 张晓[2,3] 胡文静 李曼[2] 江伟 高德荣[1,2,3] LIU Qiao;FANG Zheng-wu;ZHANG Xiao;HU Wen-jing;LI Man;JIANG Wei;GAO De-rong(College of Agronomy,Yangtze University,Jingzhou 434025,China;Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/Key Laboratory of Wheat Biology and Genetic Breeding in the Middle and Lower Reaches of Yangtze River,Ministry of Agriculture and Rural Affairs,Yangzhou 225007,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]长江大学农学院,湖北荆州434025 [2]江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007 [3]扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《江苏农业学报》2021年第2期273-279,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0100502);国家自然科学基金项目(31901544);国家现代农业产业技术体系建设专项(CARS-03-03B、CARS-3-2-11);江苏省现代农业重点项目(BE2017340);江苏省重点研发计划项目(BE2018350);江苏省自然科学基金项目(BK20171279)。

摘　　要：为了发掘更多与小麦溶剂保持力(Solvent retention capacity,SRC)显著相关的位点,以171个小麦品种(系)组成的自然群体为材料,于2017-2018和2018-2019年度分别在扬州、高邮种植,收获后测定5%乳酸SRC、5%碳酸钠SRC、50%蔗糖SRC和水SRC,结合群体90K SNP芯片基因型资料对小麦溶剂保持力进行全基因组关联分析(Genome-wide association study,GWAS)。在2年2点共4个环境下检测到12个稳定的显著性关联位点,分别位于1B、2A、4D染色体上,单个位点的表型解释率为6.93%~14.83%。在1B染色体上检测到同时控制水SRC、乳酸SRC和碳酸钠SRC的位点,可解释6.93%~14.83%表型变异率;在4D染色体上检测到同时控制水SRC、碳酸钠SRC的位点,可解释表型变异率7.04%~9.76%。发掘到的与小麦溶剂保持力显著相关的位点及其SNP可用于软质或弱筋小麦品质育种。Natural population of wheats composed of 171 varieties(strains)planted in Yangzhou and Gaoyou in 2017-2018 and 2018-2019 respectively were used as the materials to explore more sites significantly related to solvent retention capacity(SRC)of wheats.After harvest,5%lactic acid SRC,5%sodium carbonate SRC,50%sucrose SRC and water SRC were determined,and genome-wide association study(GWAS)was conducted to study the solvent retention capacity of wheat based on the genotype data of 90K SNP microarray.A total of 12 stable and significant associated loci were detected in four environments at two points of two years,which were located on chromosomes 1B,2A and 4D,respectively.The phenotypic explanation ratio of single locus was 6.93%-14.83%.The locus associated with simultaneous control of water SRC,lactic acid SRC and sodium carbonate SRC was detected in 1B chromosome,which could explain 6.93%-14.83%of the phenotypic variations.The locus associated with simultaneous control of water SRC and sodium carbonate SRC was detected in 4D chromosome,which could explain 7.04%-9.76%of the phenotypic variations.The loci and SNPs significantly related to the solvent retention capacity of wheat found in this study can be applied in the breeding of wheat with soft or weak-gluten qualities.

关 键 词：普通小麦 溶剂保持力 关联分析 弱筋小麦品质育种 

分 类 号：S512.1[农业科学—作物学]