基于二氧化硅、百草枯、博莱霉素建立3种大鼠肺纤维模型的差异蛋白质组学分析  

Differential proteomics analysis of the lung tissues in three rat pulmonary fibrosis models induced by silicon dioxide,paraquat or bleomycin

作　　者：肖梦珂 贾岩龙[2] XIAO Mengke;JIA Yanlong(College of Public Health,Xinxiang Medical University,Xinxiang 453003,China;College of Pharmacy,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院公共卫生学院,河南新乡453003 [2]新乡医学院药学院,河南新乡453003

出　　处：《肿瘤基础与临床》2021年第1期5-10,共6页journal of basic and clinical oncology

基　　金：河南省科技攻关项目(192102310149)。

摘　　要：目的以二氧化硅、百草枯、博莱霉素诱导的大鼠肺纤维化(PF)模型为研究对象,以期在3种大鼠PF模型中探究存在差异表达的参与PF发生、发展相关的关键蛋白。方法应用同位素标记相对与绝对定量技术(iTRAQ)结合液相色谱-串联质谱(LC-MS/MS)分析3种大鼠PF模型肺组织蛋白表达谱;采用Proteome Discoverer软件系统分析研究3种大鼠PF模型间蛋白表达水平的差异。结果成功构建3种大鼠PF模型。通过3种大鼠PF模型的蛋白质组学分析比较,发现共同的差异表达蛋白有57个,其中上调表达蛋白21个,下调表达蛋白36个,差异均有统计学意义(P均<0.05)。结论差异蛋白主要与细胞外基质受体相互作用、免疫应答、信号转导等途径相关,该结果将为深入探讨PF的形成、发生和发展机制奠定重要的实验和理论基础。Objective To explore differentially expressed proteins of the lung tissues in three rat pulmonary fibrosis(PF)models induced by silicon dioxide,paraquat or bleomycin.Methods The isobaric tags for relative and absolute quantification(iTRAQ)combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS)were used to identify the protein profiles of the lung tissues in the three rat PF models. Then, the differences of the protein expression levels were analyzed by using Proteome Discoverer software system. Results Three kinds of rat PF models were successfully established by using silicon dioxide, paraquat or bleomycin. Through proteomics analysis, 57 differentially expressed proteins in the three rat PF models were found, 21 proteins were up-regulated and 36 proteins were down-regulated, and the differences were statistically significant ( P <0.05). Conclusion The obtained differentially expressed proteins are mainly related to the interaction of extracellular matrix receptors, immune response and signal transduction. The findings will lay the important experimental and theoretical foundation for the discussion of the formation, occurrence and development mechanism of pulmonary fibrosis.

关 键 词：二氧化硅 百草枯 博莱霉素 同位素标记相对与绝对定量技术 

分 类 号：R730.2[医药卫生—肿瘤]