生长激素受体与肝细胞癌增殖迁移及预后的关系  

Relationship between growth hormone receptor and proliferation, migration and clinical prognosis of hepatocellular carcinoma

作　　者：钟昭众 熊志勇[1] 梁豪[1] 袁峰 曹明波 向莎 邓美海[1] Zhong Zhaozhong;Xiong Zhiyong;Liang Hao;Yuan Feng;Cao Mingbo;Xiang Sha;Deng Meihai(Department of Hepatobiliary Surgery,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院肝胆外科,广州510630

出　　处：《中华肝脏外科手术学电子杂志》2021年第2期210-214,共5页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：广东省自然科学基金(2016A030313848)。

摘　　要：目的探讨生长激素受体(GHR)在肝细胞癌(HCC)中表达及其对HCC细胞增殖、迁移能力及患者预后的影响。方法从癌症基因组图谱(TCGA)公共数据库下载HCC的RNA表达谱数据和临床信息,比较HCC组织和癌旁组织GHR mRNA表达。根据GHR表达高低绘制Kaplan-Meier生存曲线,生存分析采用Log-rank检验。qRT-PCR检测30例HCC组织和癌旁组织GHR mRNA表达。采用siRNA技术沉默HCC细胞中的GHR基因,EdU法检测siRNA组和对照组肝癌细胞的增殖能力,划痕实验观察两组细胞的迁移能力。GHR mRNA表达等数据比较采用t检验。结果 TCGA数据库分析发现,HCC组织中GHR mRNA的平均相对表达量为17.22±0.09,明显低于癌旁组织的20.26±0.12(t=-11.95,P<0.05)。30例HCC的GHR mRNA相对表达量为125.40±70.70,明显低于癌旁组织的610.20±167.73(t=-5.53,P<0.05)。生存分析显示,GHR高表达组患者的总体生存预后更好(HR=0.56,P<0.05)。97L细胞转染 siRNA 后,siRNA组GHR mRNA相对表达量为0.012 1±0.002 5,明显低于对照组的1.000 0(t=-1 342.00,P<0.05)。EdU增殖实验显示,siRNA组增殖期细胞百分率为(46.20±0.10)%,明显高于对照组的(32.50±0.04)%(t=5.36,P<0.05)。细胞划痕实验显示,沉默GHR基因后细胞的侵袭转移能力增强。结论 GHR在HCC中低表达,GHR表达与HCC患者预后相关,低表达患者预后差。抑制GHR表达可促进HCC细胞增殖和迁移。Objective To investigate the expression level of growth hormone receptor(GHR)in hepatocellular carcinoma(HCC)and its effect on the proliferation,migration of HCC cells and the clinical prognosis of HCC patients.Methods The RNA expression profile and clinical data of HCC were downloaded from the public database the Cancer Genome Atlas(TCGA),and the expression levels of GHR mRNA were compared between HCC and adjacent tissues.The survival curve was delineated by Kaplan-Meier method according to GHR expression level.The survival analysis was conducted by Log-rank test.The expression of GHR mRNA in 30 paired HCC and adjacent tissues were detected by qRT-PCR.The GHR gene in HCC cells was silenced by siRNA.The proliferation ability of HCC cells in siRNA and control groups was detected by EdU proliferation assay.The migration ability of HCC cells in two groups was observed by cell scratch test.GHR mRNA expression and other data were statistically compared by t test.Results The TCGA database analysis demonstrated that the average relative expression level of GHR mRNA in HCC tissues was 17.22±0.09,significantly lower than 20.26±0.12 in adjacent tissues(t=-11.95,P<0.05).The relative expression of GHR mRNA in 30 HCC tissues was 125.40±70.70,significantly lower than 610.20±167.73 in adjacent tissues(t=-5.53,P<0.05).Survival analysis showed that patients with high expression of GHR had better overall survival and prognosis(HR=0.56,P<0.05).After 97L cells were transfected with siRNA,the relative expression of GHR mRNA in siRNA group was 0.0121±0.0025,significantly lower than1.0000 in control group(t=-1342.00,P<0.05).EdU proliferation assay showed that the percentage of cells in proliferation phase in siRNA group was(46.20±0.10)%,significantly higher than(32.50±0.04)%in the control group(t=5.36,P<0.05).Cell scratch test revealed that the invasion and metastasis abilities of cells enhanced after silencing the GHR gene.Conclusions The expression level of GHR is low in HCC,which is associated with the clinical prognosis of H

关 键 词：癌 肝细胞 生长激素受体 细胞增殖 细胞迁移 预后 

分 类 号：R735.7[医药卫生—肿瘤]