长链非编码RNA LINC00520调控IGF2BP1介导肺腺癌细胞对紫杉醇化疗耐药的影响  

LINC00520 mediates drug resistance of lung adenocarcinoma cells to paclitaxel chemotherapy by regulating IGF2BP1

作　　者：刘海君[1] 赵俊刚[1] LIU Haijun;ZHAO Jungang(Department of Thoracic Surgery,Affiliated Shengjing Hospital of China Medical University,Shenyang,Liaoning 110004,China)

机构地区：[1]中国医科大学附属盛京医院胸外科,沈阳110004

出　　处：《重庆医学》2021年第8期1266-1271,共6页Chongqing medicine

基　　金：辽宁省自然科学基金项目(20170540562)。

摘　　要：目的观察长链非编码RNA LINC00520对肺腺癌(LUAD)增殖、凋亡和对紫杉醇化疗耐药的影响,并探讨其机制。方法实时荧光聚合酶链反应(RT-PCR)检测人支气管上皮细胞株(BEAS-2B)和LUAD细胞中LINC00520的表达水平;生物信息学分析获取LINC00520相关结合蛋白;在LUAD中抑制LINC00520表达后,RT-PCR、Western blot检测胰岛素样生长因子ⅡmRNA结合蛋白1(IGF2BP1)的表达水平;在LUAD细胞中抑制LINC00520后,再过表达IGF2BP1,应用CCK-8和Annexin V/PI双染评估细胞增殖和凋亡,以及对紫杉醇治疗的影响。结果与BEAS-2B相比,LUAD细胞中LINC00520的表达水平明显升高(P<0.05);IGF2BP1是LINC00520潜在的结合蛋白;抑制LINC00520表达后LUAD细胞中IGF2BP1的表达下调;LUAD细胞敲降LINC00520后,细胞增殖减少、凋亡增多,紫杉醇化疗敏感性增加(P<0.05),而过表达IGF2BP1后上述表现明显恢复(P<0.05)。结论LINC00520可通过上调IGF2BP1的表达促进LUAD细胞紫杉醇化疗耐药。Objective To observe the effect of long non-coding RNA LINC00520 on the proliferation,apoptosis and paclitaxel chemotherapy resistance of lung adenocarcinoma(LUAD),and to investigate its mechanism.Methods The expression of LINC00520 in bronchial epithelial cell line(BEAS-2B)and LUAD cells was detected by real-time fluorescent quantitative PCR(RT-PCR);the bioinformatics analysis was performed to obtain the LINC00520 associated binding protein;the expression level of IGF2BP1 after inhibiting LINC00520 expression in LUAD cells was examined by RT-PCR and Western-blot;the cellular proliferation,apoptosis and effect on paclitaxel treatment after inhibiting LINC00520 and overexpressing IGF2BP1 were examined by CCK-8 and Annexin V/PI double staining.Results The expression level of LINC00520 in LUAD cells was significantly increased as compared with those in BEAS-2B cells(P<0.05).IGF2BP1 was a potential binding protein of LINC00520.After inhibiting LINC00520 expression,the IGF2BP1 expression in LUAD cells;after LUAD cells knocking down LINC00520,the cellular proliferation was decreased,apoptosis was enhanced and the paclitaxel chemotherapy was sensitive(P<0.05),while overexpressing IGF2BP1,the above phenotype was significantly recovered(P<0.05).Conclusion LINC00520 promotes the paclitaxel chemotherapy resistance of LUAD cells by upregulating the expression of IGF2BP1.

关 键 词：肺腺肿瘤 LINC00520 胰岛素样生长因子ⅡmRNA结合蛋白1 细胞增殖 细胞凋亡 化疗药物 耐药性 紫杉醇 

分 类 号：R734.2[医药卫生—肿瘤]