多菌灵降解菌djl-6和啶虫脒降解菌D-2固体菌剂的研发  

Study of Solid Bacterial Agents for Carbendazim-degrading Strain djl-6 and Acetamipriddegrading Strain D-2

作　　者：徐铭阳 卢家森 孟献雨 林泽彬 何健[1] 黄星[1] XU Mingyang;LU Jiasen;MENG Xianyu;LIN Zebin;HE Jian;HUANG Xing(College of Life Science,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学生命科学学院,南京210095

出　　处：《土壤》2021年第2期329-335,共7页Soils

基　　金：国家重点研发计划项目(2016YFD0801102);江苏省农业科技自主创新资金项目(CX(18)1005);江苏省高等学校大学生实践创新训练计划项目(201810307012)资助。

摘　　要：以蚯蚓粪肥、猪粪肥、鸡粪肥、秸秆肥、米糠、泥炭以及花生壳粉作为载体,以多菌灵降解菌Rhodococcus qingshengii djl-6和啶虫脒降解菌Pigmentiphaga sp.D-2为材料,开展了固体菌剂的研发。接种菌株djl-6及D-2后,各载体均能够促进供试菌株的生长。以蚯蚓粪肥作为载体时,菌株djl-6、D-2有效菌数释放率最高,分别为100.40%和82.03%。在保存120 d时,除花生壳粉外,其余各载体中的活菌数均高于10^(7) cfu/g;以蚯蚓粪为载体的djl-6菌剂活菌数达到了7.00×10^(8) cfu/g,以猪粪为载体的D-2菌剂活菌数达到了4.29×10^(8) cfu/g。固体菌剂保存30 d后进行土壤农药残留降解试验,以蚯蚓粪为载体的djl-6菌剂对5 mg/kg的多菌灵降解率为94.30%,以蚯蚓粪为载体的D-2菌剂对10 mg/kg的啶虫脒降解率为81.87%。研究结果表明,以蚯蚓粪为载体的固体农残降解菌剂活菌数高、保存期长,具有较好的应用前景。In this study,earthworm manure,pig manure,chicken manure,straw manure,rice bran,peat,and peanut shell powder were used as carriers.The carbendazim-degrading bacteria Rhodococcus qingshengii djl-6 and the acetamiprid-degrading bacteria Pigmentiphaga sp.D-2 were used as materials.After being inoculated the strains djl-6 and D-2,all carriers could promote the growth of the tested strains.When earthworm manure was used as the carrier,the effective release rates of strains djl-6 and D-2 were the highest,reaching 100.40%and 82.03%respectively.When stored for 120 d,the number of viable bacteria in each carrier except peanut shell powder was higher than 10^(7) cfu/g.The live bacterial count of djl-6 bacterial agent using earthworm manure as a carrier reached 7.00×10^(8) cfu/g,and the live bacterial count of D-2 bacterial agent using pig manure as a carrier reached 4.29×10^(8) cfu/g.After the solid bacterial agent was stored for 30 d,the soil pesticide residue degradation test was performed.The djl-6 bacteria agent with earthworm manure as a carrier degraded 94.30%of carbendazim at 5 mg/kg,and the D-2 bacteria with earthworm manure as a carrier degraded 81.87%of carbendazim at 10 mg/kg.The results of this study indicated that the solid pesticide residue degrading bacterial agent using earthworm manure as a carrier has a high number of live bacteria and a long shelf life,and has a good application prospect.

关 键 词：多菌灵降解菌djl-6 啶虫脒降解菌D-2 固体菌剂 

分 类 号：X172[环境科学与工程—环境科学]