Enhancing the Surface Properties of a Bioengineered Anterior Cruciate Ligament Matrix for Use with Point-of-Care Stem Cell Therapy  

作　　者：Xiaohua Yu Paulos Y.Mengsteab Ganesh Narayanan Lakshmi S.Nair Cato T.Laurencin 

机构地区：[1]Connecticut Convergence Institute for Translation in Regenerative Engineering,University of Connecticut Health,Farmington,CT 06030,USA [2]Raymond and Beverly Sackler Center for Biological,Physical and Engineering Sciences,University of Connecticut Health,Farmington,CT 06030,USA [3]Department of Orthopedic Surgery,University of Connecticut Health,Farmington,CT 06030,USA [4]Department of Biomedical Engineering,University of Connecticut,Storrs,CT 06269,USA [5]Department of Materials Science and Engineering,University of Connecticut,Storrs,CT 06269,USA [6]Department of Chemical and Biomolecular Engineering,University of Connecticut,Storrs,CT 06269,USA [7]Department of Reconstructive Sciences,University of Connecticut Health,Farmington,CT 06030,USA

出　　处：《Engineering》2021年第2期153-161,共9页工程（英文）

基　　金：This research was supported by funding from the Raymond and Beverly Sackler Center for Biomedical,Biological,Physical and Engineering Sciences(NIH R01AR063698,NIH R01AR063698-02S1,and NIH DP1 AR068147).

摘　　要：We have previously developed a poly(L-lactic)acid(PLLA)bioengineered anterior cruciate ligament(ACL)matrix that has demonstrated enhanced healing when seeded with primary ACL cells prior to implantation in a rabbit model,as compared with the matrix alone.This suggests that improving cell adhesion on the matrix may beneficially affect the healing response and long-term performance of the bioengineered ACL matrix.One regenerative engineering approach involves enhancing the surface properties of the matrix to support cell adhesion and growth in combination with point-of-care stem cell therapy.Herein,we studied the cell adhesion properties of PLLA braided microfiber matrices enhanced through the physical adsorption of fibronectin and air plasma treatment.We evaluated the kinetics and binding efficiency of fibronectin onto matrices at three time points and three fibronectin concentrations.Incubating the matrix for 120 min in a solution of 25 lgmL1 fibronectin achieved the greatest binding efficiency to the matrix and cellular adhesion.Exposing the matrices to air plasma treatment for 5 min before fibronectin adsorption significantly enhanced the cell adhesion of rabbit bone marrow-derived mesenchymal stem cells(R-BMMSCs)24 h post cell seeding.Finally,cellular proliferation was monitored for up to 21 d,the matrices were exposed to air plasma treatment,and fibronectin adsorption was found to result in enhanced cell number.These findings suggest that exposure to air plasma treatment and fibronectin adsorption enhances the cellular adhesion of PLLA braided microfiber matrices and may improve the clinical efficacy of the matrix in combination with point-of-care stem cell therapies.

关 键 词：Anterior cruciate ligament LIGAMENT Poly(L-lactic)acid plasma treatment FIBRONECTIN Stem cells ADHESION 

分 类 号：R318[医药卫生—生物医学工程]