肌苷对精神分裂症小鼠认知能力及海马超微结构的影响  

The effects of inosine on the cognitive ability and ultrastructure of hippocampus in mice with schizophrenia

作　　者：李依璐 马文[2] 陈小江[1] 孙金萍[2] 王俊燕 王聃 刘娟[1] 马全瑞[1] Li Yilu;Ma Wen;Chen Xiaojiang;Sun Jinping;Wang Junyan;Wang Dan;Liu Juan;Ma Quanrui(College of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China;General Hospital,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学基础医学院,银川750004 [2]宁夏医科大学总医院,银川750004

出　　处：《神经解剖学杂志》2021年第2期152-156,共5页Chinese Journal of Neuroanatomy

基　　金：宁夏自然科学基金(NZ17072)。

摘　　要：目的:探究口服肌苷对精神分裂症(SP)小鼠模型学习记忆能力及海马神经元和髓鞘超微结构的作用。方法:30只ICR雄性小鼠按照随机性分为对照组(control)、模型组(MK-801)以及肌苷治疗组(Inosine+MK-801),每组10只。连续14d腹腔注射MK-801制备SP模型,Inosine+MK-801组小鼠于制备模型前30 min给予肌苷灌胃治疗并持续治疗16d。Morris水迷宫观察肌苷治疗S P模型小鼠后的行为学改变,透射电子显微镜观察海马髓鞘、神经元超微结构的变化。结果:Morris水迷宫结果显示:与control组相比较,MK-801组小鼠的逃避潜伏时间明显增加,经过平台所在位置的次数明显降低(P<0.01);Inosine+MK-801组与MK-801组比较,Inosine+MK-801组的逃避潜伏时间明显减少,经过平台所在位置的次数明显增多(P<0.05)。透射电镜图像结果示control组小鼠海马髓鞘形态规则、结构致密完整、板层清晰可见、无水肿,神经元核膜完整规则、染色质均匀、各细胞器形态规则完整并清晰可见;MK-801组小鼠海马内的髓鞘形态不规则且出现髓鞘局灶性溶解、板层结构模糊不清,神经元核膜不完整、胞质局部性空化和水肿,内质网减少;Inosine+MK-801组髓鞘和神经元超微结构损伤明显改善。结论:肌苷治疗可提高SP小鼠学习记忆能力、改善SP小鼠海马髓鞘和神经元的超微结构损伤。Objective:To explore the effects of inosine on the learning and memory ability and ultrastructure of hippocampal neurons and myelin sheath in schizophrenia(SP)mice models.Methods:Thirty male ICR mice were randomly divided into normal control group(control),model group(MK-801),inosine treatment group(Inosine+MK-801),with 10 mice in each group.The SP mice models were acquired by intraperitoneal injection of MK-801 for 14 days.Thirty minutes before the preparation of the model,the mice in the Inosine+MK-801 group were given inosine by intragastric administration for 16 days.Morris water maze and transmission electron microscopy were used to observe the changes of behavior and the ultrastructure of hippocampal neurons and myelin sheath in SP mice after being treated with inosine.Results:Morris water maze results showed that the escape latency time was longer,and the times of passing through the platform was significantly reduced(P<0.01)in the MK-801 group to compare with the control group.The escape latency time of the Inosine+MK-801 group was reduced more significantly,and the times of passing through the platform position was increased more significantly than the MK-801 group(P<0.05).The results of transmission electron microscope experiments showed that the myelin sheathes were regular and intact,the lamellar layers of myelin sheath were clear and no edema,the nuclear membrane of neurons was intact and regular,the nucleoplasm and chromatin of neurons were uniform,the organelles morphology of neurons were regular and clear in the hippocampus of control group;The myelin sheathes were irregular and regionally dissolved,the lamellar layers of myelin sheath were vague,the nuclear membrane of neurons was incomplete,the neuronal cytoplasm was cavitating and edematous,the number of endoplasmic reticulum in neurons was reduced in the hippocampus of MK-801 group;The damage of myelin sheath and neuronal ultrastructures in the Inosine+MK-801 group were significantly improved.Conclusion:Inosine treatment can enhance the learni

关 键 词：肌苷 精神分裂症 海马 髓鞘 神经元 小鼠 

分 类 号：R749.3[医药卫生—神经病学与精神病学]