免疫组织化学法与荧光原位杂交技术检测861例乳腺癌HER-2表达的一致性分析  

The evaluation of HER-2 status in 861 cases of breast cancer patients by IHC and FISH

作　　者：王清 袁勇 陆建荣 丁彩霞 张娟[2] 王晓敏[2] 任栋栋 WANG Qing;YUAN Yong;LU Jianrong;DING Caixia;ZHANG Juan;WANG Xiaomin;REN Dongdong(Xi'an Medical University,Shaanxi Xi'an 710068,China;Department of Pathology,Shaanxi Provincial Cancer Hospital Affiliated to Xi'an Jiaotong University,Shaanxi Xi'an 710061,China)

机构地区：[1]西安医学院,陕西西安710068 [2]西安交通大学附属陕西省肿瘤医院病理科,陕西西安710061

出　　处：《现代肿瘤医学》2021年第10期1672-1676,共5页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:2014JM4187)。

摘　　要：目的:对比免疫组织化学法(IHC)与荧光原位杂交技术(FISH)检测861例乳腺癌人类表皮生长因子受体2(HER-2)蛋白表达和基因状态的一致性。方法:参照《乳腺癌HER-2检测指南(2019版)》判读标准对乳腺癌石蜡组织采用IHC法及FISH法分别检测HER-2蛋白表达和HER-2基因状态,进行比较分析。结果:861例乳腺癌患者中,IHC HER-2(0/1+)和FISH(-)的一致率为97.1%(134/138);IHC HER-2(2+)和FISH(+)的一致率为28.7%(188/655);IHC HER-2(3+)和FISH(+)的一致率为98.5%(67/68)。两种检测方法的检测结果存在一致性,差异具有统计学意义(Kappa=0.137,P<0.0001)。结论:两种方法相结合,以便精准评价HER-2检测结果指导靶向治疗。Objective:To evaluate the concordance of HER-2/neu protein expression and gene status by immunohistochemistry(IHC)and fluorescence in situ hybridisation(FISH)in 861 cases of breast cancer patients.Methods:Referring to the guideline for HER-2 detection in breast cancer,the 2019 version,the protein expression and gene status of HER-2 in paraffin-embeded tissues of breast cancer were detected by IHC and FISH,respectively,and the concordance was evaluated.Results:Among 861 cases of breast cancer patients,the coincidence rate of IHC(0/1+)and FISH(-)was 97.1%(134/138).The coincidence rates of IHC(2+/3+)and FISH(+)were 28.7%(188/655)and 98.5%(67/68),respectively.The Kappa test result showed there was concordance between IHC and FISH(Kappa=0.137,P<0.0001).Conclusion:The combination of IHC and FISH should evaluate accurately the expression of HER-2 and guide targeting therapy.

关 键 词：乳腺癌 人类表皮生长因子受体2 免疫组织化学法 荧光原位杂交技术 

分 类 号：R737.9[医药卫生—肿瘤]