环境条件对温和气单胞菌群体感应基因luxI/luxR表达的影响  

Effects of Environmental Conditions on Gene luxI/luxR Expression of Aeromonas sobria Based on Quorum Sensing System

作　　者：孙晓佳 李婷婷 赫彬彬 梅永超 谢晶[3] 励建荣 Sun Xiaojia;Li Tingting;He Binbin;Mei Yongchao;Xie Jing;Li Jianrong(College of Food Science and Technology,Bohai University,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province,The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities,Jinzhou 121013,Liaoning;College of Life Science,Dalian Minzu University,Dalian 116600,Liaoning;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁省食品安全重点实验室,辽宁省高等学校生鲜食品产业技术研究院,辽宁锦州121013 [2]大连民族大学生命科学学院,辽宁大连116600 [3]上海海洋大学食品学院,上海201306

出　　处：《中国食品学报》2021年第4期71-78,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划重点专项(2018YFD 0400600,2017YFD0400106)。

摘　　要：以大菱鲆源温和气单胞菌群体感应调控基因luxI/luxR为研究对象,采用实时荧光定量PCR技术从转录水平上探究环境条件对该基因表达的影响,以期确定环境因素对群体感应系统的调节作用。结果表明:在适当低温(15℃)及弱碱(pH=8)环境条件下,luxI/luxR基因表达水平最高;而在高渗透压胁迫环境条件下,2种基因的表达水平明显下降。当葡萄糖为碳源时,luxI/luxR基因的表达量最高,且luxI基因对碳源的敏感程度高于luxR基因。不同氮源培养条件下luxI基因表达情况依次为胰蛋白胨>氯化铵>牛肉膏>酵母浸粉>脲素,而luxR基因表达情况依次为胰蛋白胨>氯化铵>酵母浸粉>牛肉膏>脲素。可以通过改变环境条件调节温和气单胞菌的群体感应系统,从而达到控制其致腐能力的目的。Quorum sensing gene luxI/luxR of Aeromonas sobria was used for this study,and the effect of different environmental conditions on the expression of this gene was investigated from the transcription level by real-time fluorescence quantitative PCR to determine the regulatory effect of environmental factors on quorum sensing system.The results showed that the expression level of luxI/luxR gene was the highest at appropriate low temperature(15℃)and weak base(pH=8),while the expression level of both genes decreased under high osmotic stress.When glucose was the carbon source,the expression of luxI/luxR gene was the highest,and luxI gene was more sensitive to carbon sources than luxR gene.The expression level of luxI gene under different nitrogen sources was as follows:tryptone>ammonium chloride>beef extract>yeast extract>urea,and the expression of luxR gene was as follows:tryptone>ammonium chloride>yeast extract>beef extract>urea.Therefore,the changes of environmental conditions can be used to regulate quorum sensing system in Aeromonas sobria,so as to control its spoilage ability.

关 键 词：温和气单胞菌 环境条件 群体感应 实时荧光定量PCR 

分 类 号：S941.4[农业科学—水产养殖]