亚甲蓝通过诱导巨噬细胞极化发挥预防脓毒症肝损伤作用  

Methylene blue play a role in preventing septic liver injury by inducing macrophage polarization

作　　者：李晗 田李均[2] 卞兆连[3] 陈强 施玲灵 韩旭东[2] Li Han;Tian Lijun;Bian Zhaolian;Chen Qiang;Shi Lingling;Han Xudong(Medical School of Nantong University,Nantong 226001,China;Intensive Care Unit,Nantong Third People’s Hospital,Nantong 226000,China;Department of Gastroenterology,Nantong Third People’s Hospital,Nantong 226000,China)

机构地区：[1]南通大学医学院,226001 [2]南通市第三人民医院重症医学科,226000 [3]南通市第三人民医院消化内科,226000

出　　处：《中华肝脏病杂志》2021年第4期369-372,共4页Chinese Journal of Hepatology

基　　金：南通市卫健委市级重点学科支持项目(wx2017002);南通市科技计划项目(JC2020054、MS12018040、MS22018007);南通市卫健委科研课题(QA2019033、MA2020017)。

摘　　要：30只小鼠采用盲肠结扎穿刺法制作脓毒症模型,腹腔注射15 mg/kg亚甲蓝或等渗盐水。观察肝组织病理改变,检测肝巨噬细胞频数变化和肝M1、M2型炎症因子表达情况,检测脂多糖刺激后巨噬细胞炎症因子表达水平。结果显示MB组小鼠的肝病理学损伤明显减轻(P<0.05);肝巨噬细胞频数变化差异无统计学意义(P>0.05);MB促进肝M2型炎症因子表达升高(P<0.05)、巨噬细胞M2型炎症因子表达增多(P<0.05)。MB可通过诱导巨噬细胞向M2型极化从而发挥预防脓毒症肝损伤的作用。Thirty mice were used to establish a sepsis model with cecal ligation and puncture.15 mg/kg methylene blue or isotonic saline were injected intraperitoneally to observe liver tissue pathological changes.Changes in macrophage frequency and expressional condition of M1 and M2-type hepatic inflammatory factors were detected.After LPS stimulation,the expression level of macrophage inflammatory factor were detected.The results showed that the pathological liver injury was significantly reduced in the MB mice group(P<0.05),and the frequency of liver macrophage was not statistically significantly different(P>0.05).MB elevation had promoted the expression of M2-type hepatic inflammatory factor(P<0.05)and macrophage inflammatory factor(P<0.05).MB can play a role in preventing septic liver injury by inducing macrophages polarization to M2-type.

关 键 词：亚甲蓝 脓毒症 肝损伤 巨噬细胞 

分 类 号：R575[医药卫生—消化系统] R459.7[医药卫生—内科学]