罕见的A3亚型的血清学和分子生物学研究  

Serological and molecular biological studies of rare A3 subtype

作　　者：李茵[1] 赵倩[1] 苏蔓[1] 钱明明[1] 乔芳[1] 王远花[1] 郭霞[1] 王振雷[1] LI Yin;ZHAO Qian;SU Man;QIAN Mingming;QIAO Fang;WANG Yuanhua;GUO Xia;WANG Zhenlei(Hebei Blood Center,Shijiazhuang,050000,China)

机构地区：[1]河北省血液中心配型中心,石家庄050000

出　　处：《临床血液学杂志》2021年第2期109-111,共3页Journal of Clinical Hematology

摘　　要：目的:对罕见的A3亚型进行血清学检测和ABO基因测序,探讨其分子生物学特性以及血清型表型和基因型的关系。方法:应用常规血清学定型方法进行ABO正反定型;对4例正反定型不符样本的第6、7外显子运用PCR扩增后直接测序,确定其基因型。结果:3例血清学表型为A亚B,1例为A亚的样本其中一条单链序列与ABO*A1.01比对,发现其在467位和745位碱基均存在C>T的突变。结论:血清学和直接测序结果表明,此4例标本均包含罕见的ABO*A3.07等位基因。Objective: To study the relationship between serotype phenotype and genotype by detecting the rare A3 subtype using serology and ABO gene sequencing. Methods: The forward and reverse ABO typing was carried out by routine serological typing. For the samples inconsistent with forward and reverse ABO typing, their exon 6 and exon 7 were amplified by PCR and sequenced directly to determine the genotypes. Results: Among the 4 samples, 3 cases were A sub-B serological phenotype, and 1 was A sub serological phenotype. One single strand sequence was compared with ABO*A1.01 and it was found that there was mutation of C>T at 467 and 745 sites in all four samples. Conclusion: The results of serology and direct sequencing showed that all the 4 samples contained rare ABO*A3.07 allele.

关 键 词：ABO亚型 A3亚型 血清型表型 基因测序 

分 类 号：R343.1[医药卫生—基础医学]