高效液相色谱法同时测定化妆品中9种4-羟基苯甲酸酯类防腐剂  

Simultaneous determination of nine kinds of 4-hydroxybenzoate preservatives in cosmetics by high performance liquid chromatography

作　　者：邢海艳 孙丽 吴旭[1] 鲁素雅 金鹏[1] 金华[1] Xing Haiyan;Sun Li;Wu Xu;Lu Suya;Jin Peng;Jin Hua(Huai’an Institute for Food and Drug Control,Huai’an 223005,China)

机构地区：[1]淮安市食品药品检验所,江苏淮安223005

出　　处：《化学分析计量》2021年第5期46-50,共5页Chemical Analysis And Meterage

摘　　要：建立同时测定化妆品中9种4-羟基苯甲酸酯类防腐剂的高效液相色谱法。样品经甲醇溶解,超声提取,使用Waters Symmtery C_(18)(2.1 mm×250 mm,5μm)色谱柱,用0.1%磷酸溶液–乙腈为流动相进行梯度洗脱,采用二极管阵列检测器进行检测,检测波长为254 nm,外标法定量。9种防腐剂的质量浓度在0.1~100μg/mL范围内与对应的色谱峰面积线性关系良好,相关系数为0.9993~0.9997,检出限为0.65~2.22μg/g,定量限为2.18~7.39μg/g。样品在25、50、250μg/g 3个加标水平时的平均回收率为95.73~104.06%,测定结果的相对标准偏差为0.58~5.28%(n=6)。该方法简单、准确、灵敏,可用于化妆品中9种4-羟基苯甲酸酯类防腐剂的测定。A method for simultaneous determining of nine kinds of 4-hydroxybenzoate preservatives in cosmetics by high performance liquid chromatography was established.The sample was dissolved in methanol and extracted by ultrasound.Waters Symmtery C_(18)(2.1 mm×250 mm,5μm)column was used for gradient elution with 0.1%phosphoric acid solution–acetonitrile as mobile phase.Diode array detector was used for detection,the detection wavelength was 254 nm and the external standard method was used for quantitative analysis.The mass concentration of nine preservatives had a good linear relationship with the chromatographic peak area in the range of 0.1–100μg/mL,the correlation coefficient was 0.9993–0.9997,the detection limits were 0.65–2.22μg/g and the quantification limits were 2.18–7.39μg/g.The average recoveries of standard addition in three different levels of 25,50 and 250μg/g were 95.73–104.06%,and the relative standard deviations of the determination results were 0.58–5.28%(n=6).The method is simple,accurate and sensitive,which can be used for the determination of nine kinds of 4-hydroxybenzoate preservatives in cosmetics.

关 键 词：高效液相色谱法 4-羟基苯甲酸酯类 化妆品 防腐剂 

分 类 号：O657.7[理学—分析化学]