小苍兰花瓣花黄色素组成和含量分析  

Analysis of Composition and Content of Anthoxanthins in Petals of Freesia hybrida

作　　者：朱佳意 唐东芹 李欣 ZHU Jiayi;TANG Dongqin;LI Xin(School of Design,Shanghai Jiao Tong University,Shanghai 200240,China;Instrumental Analysis Center,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学设计学院,上海200240 [2]上海交通大学分析测试中心,上海200240

出　　处：《热带作物学报》2021年第4期1136-1144,共9页Chinese Journal of Tropical Crops

基　　金：上海市农委科技兴农重点攻关项目(No.G20140102)。

摘　　要：以6个不同花色的小苍兰品种为实验材料,在量化小苍兰花色表型和初步确定花瓣色素类型的基础上,利用UPLC-Q-TOF-MS技术鉴定花瓣中的花黄色素组成并比较分析各组分含量。结果表明:在小苍兰花瓣中鉴定到13种黄酮及黄酮醇物质,分别为槲皮素、山萘酚、异鼠李素、柚皮素、黄芩素及其糖苷;槲皮素及其糖苷是小苍兰花瓣中重要的黄酮醇物质;随着小苍兰的花朵开放,花黄色素含量多呈下降趋势;在不同小苍兰品种中,花黄色素含量存在明显差异,其中,红、紫色系品种含量显著高于黄、白色系品种,尤其是‘上农红台阁’‘Red Passion’和‘Castor’,其含量在各发育阶段均表现为较高水平的积累。此外,花色表型为复色的‘Red Passion’和‘Castor’中上部与基部花瓣的花黄色素含量存在显著差异。相关分析表明,各花黄色素组分的积累与小苍兰花色明度均呈极显著负相关;槲皮素、异鼠李素及其糖苷含量与花色红度呈极显著正相关,柚皮素、山萘酚及其糖苷与花色红度呈显著正相关;山萘酚及其糖苷含量与花色鲜艳程度呈显著正相关。可见,花黄色素影响小苍兰的花色呈现。In this study,the petal color of Freesia hybrida was measured by the colorimetric card and color meter,then the composition and content of anthoxanthins in petals were analyzed using UPLC-Q-TOF-MS in order to explore the relationship between petal color and the anthoxanthins content.The results showed that 5 flavonoid aglycones,up to 13 flavones and flavonols,were detected in the petals of F.hybrida for the first time.Quercetin and its glycosides proved to be an important flavonol in the petals.During flowering stages,the content of anthoxanthins decreased in general.There were significant differences in the content and components of anthoxanthins among cultivars,namely the content in red and purple cultivars,especially in’SN Hongtaige’,’Red Passion’and’Castor’,were significantly higher than that in yellow and white cultivars.Significant difference in the content of anthoxanthins between the top and the base petals of’Red Passion’and’Castor’was reported.Correlation analysis showed a significant negative correlation between the accumulation of anthoxanthins and the brightness of the petals.The redness of petals showed a significantly positive correlation with quercetin and isorhamnetin glycosides,and a positive correlation with naringenin and kaempferol glycosides.The saturation of petals was positively correlated with kaempferol glycosides.Anthoxanthins proved to be closely related to flower color.

关 键 词：小苍兰 花黄色素 黄酮 黄酮醇 四级杆飞行时间串联质谱 花色 

分 类 号：Q946[生物学—植物学]