IL-10基因多态性与淋巴性白血病易感性的关系  

Relationship Between IL-10 Gene Polymorphism and Susceptibility to Lymphoblastic Leukemia

作　　者：石永军 赖晓霏[1] 杨玲[1] 林玉娟 丁敏[1] SHI Yongjun;LAI Xiaofei;YANG Ling;LIN Yujuan;DING Min(Medical Laboratory Department,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院医学检验科,重庆400016

出　　处：《中国继续医学教育》2021年第15期120-124,共5页China Continuing Medical Education

基　　金：重庆市教委科学技术研究计划项目课题(渝教科发〔2019〕14号)。

摘　　要：目的研究在重庆市地区血液病患者和健康人群中,IL-10基因启动子592位点基因多态性的分布情况及其与白血病易感性之间的关系。方法从医院住院部和体检中心分别选取各型白血病患者114例和健康体检者140例,应用PCR-限制性长度片段多态性方法,检测该研究人群中IL-10-592位点的多态性。结果ALL组IL-10-592基因型频率分别为:纯合突变型(AA)8例(18.6%),杂合子(AC)5例(11.6%),野生型(CC)30例(69.8%);CLL组IL-10-592基因型频率分别为:纯合突变型(AA)11例(15.5%),杂合子(AC)10例(14.1%),野生型(CC)50例(70.4%);就对照组而言,其IL-10-592基因型的频率是80例纯合突变型(AA),占57.1%,46例杂合子(AC),占32.9%,14例野生型(CC),占10.0%。且统计数据显示,ALL和CLL两组基因型频率与对照组比较,差异具有统计学意义(P<0.001)。多元Logistic回归结果表明,与携带IL-10-592 A/A者相比较,携带IL-10-592 C/C者发生ALL的危险性为OR=26.82(95%CI:11.30~63.64,P=0.000)。携带IL-10-592 C/C基因型者患CLL的风险是携带IL-10-592 A/A基因型者的26倍(OR=26.70,95%CI:9.72~73.30,P=0.000)。结论研究结果提示IL-10-592位点基因多态性与急慢性淋巴白血病的易感性密切相关。Objective To discuss the polymorphisms of IL-10 gene and the association with susceptibility of leukemia in Han people in Chongqing.Methods ALL 43 patients and 71 patients with CLL,and 140 healthy controls were genotyped for IL-10-592 gene polymorphisms by the PCR-RFLP method.Results The results indicated that the frequencies of IL-10-592 genotypes in leukemia group was significantly different comparing control group(P<0.001).Multivariate Logistic regression analysis showed that the risk of all in patients with il-10-592 C/C was 26.82(95%CI:11.30~63.64,P=0.000)compared with those with il-10-592 A/A.The risk of CLL in patients with il-10-592 C/C genotype was 26 times higher than that in patients with il-10-592 A/A genotype(OR=26.70,95%CI:9.72~73.30,P=0.000).Conclusion The results suggest that the polymorphism of IL-10-592 gene has close association with susceptibility of acute and chronic lymphatic leukemia.

关 键 词：白细胞介素-10 基因多态性 淋巴白血病 肿瘤 易感性 基因频率 

分 类 号：R733[医药卫生—肿瘤]