松果菊苷对大鼠抑郁模型线粒体损伤的修复作用  

Effect of echinacoside on mitochondrial damage in a rat model of depression

作　　者：岳凌峰 马敬[2] 王宁[3] YUE Lingfeng;MA Jing;WANG Ning(the Second Affiliated Hospital of Xinxiang Medical University,4th Department,Xinxiang 453002,China;the Second Affiliated Hospital of Xinxiang Medical University,Department of Mood Disorder,Xinxiang 453002;the Second Affiliated Hospital of Xinxiang Medical University,3th Department,Xinxiang 453002)

机构地区：[1]新乡医学院第二附属医院精神四科,河南新乡453002 [2]新乡医学院第二附属医院心境障碍科,河南新乡453002 [3]新乡医学院第二附属医院精神三科,河南新乡453002

出　　处：《中国比较医学杂志》2021年第4期63-69,共7页Chinese Journal of Comparative Medicine

基　　金：河南省科技攻关项目(182102310242)。

摘　　要：目的本研究旨在探索松果菊苷(EA)对抑郁症模型大鼠线粒体损伤的影响。方法斯普拉格-杜勒鼠(SD)大鼠随机分为5组:对照组、单纯EA组、模型组、治疗组和氟西汀组。采用实施慢性不可预知的轻度压力(CUMS)构建大鼠抑郁症模型。治疗组大鼠腹腔内注射EA 30 mg/(kg·d),记录大鼠体重、检测大鼠糖水偏好指数评估大鼠行为。HE染色检测脑组织损伤情况;TUNEL检测脑组织细胞凋亡情况;JC-1法检测线粒体膜电位;ELISA检测炎症因子血清中IL-6、IL-1β、TNF-α和IL-10水平;Western blot检测胞质内磷酸化线粒体动力相关蛋白(p-Drp1)蛋白的表达;试剂盒检测氧化应激标记物SOD、MDA和LDH的含量。结果与对照组相比,单纯EA组的各项检测指标差异无统计学意义。而与对照组相比,模型组大鼠表现出体重降低(P<0.01);糖水偏好降低(P<0.01);血清促炎症因子IL-6、IL-1β和TNF-α水平增加;脑组织损伤加重;p-Drp1表达减少(P<0.01);脑组织氧化应激水平增加(P<0.01);而治疗组表现出治疗作用,能回救CUMS造成的上述病理损伤。结论松果菊苷可减轻CUMS抑郁症模型大鼠线粒体损伤。Objective To investigate the effects of echinacoside(EA)on mitochondrial injury in rats with depression.Methods Sprague-Dawley rats were divided into five groups of control,EA,model,treatment,and fluoxetine groups.A rat model of depression was constructed with chronic unpredictable mild stress(CUMS).Rats in the treatment group were intraperitoneally injected with EA mg/(kg·d).The weight of the rats was recorded,and the sugar water preference index was detected to evaluate the behavior of the rats.Hematoxylin and eosin staining was used to detect damage of brain tissue.Apoptosis of brain cells was detected by TUNEL assay.The mitochondrial membrane potential was detected by JC-1 method.Serum interleukin(IL)-6,IL-1β,tumor necrosis factor-α,and IL-10(inflammatory cytokines)levels were detected by ELISA.Expression of phosphorylated mitochondrial dynamic associated protein(p-Drp1)in the cytoplasm was detected by Western blot.The content of superoxide dismutase,malondialdehyde,and lactate dehydrogenase,which are markers of oxidative stress,was detected by kits.Results There were no significant differences in the detection index in the EA group compared with the control group.The model group showed weight loss compared with the control group(P<0.01).Sugar water preference was lower(P<0.01),serum levels of the pro-inflammatory cytokines IL-6,IL-1β,and tumor necrosis factor-αwere higher,and levels of the anti-inflammatory cytokine IL-10 were lower in the EA group than in the control group(all P<0.01).Brain tissue injury was aggravated,p-Drp1 expression was lower(P<0.01),and oxidative stress in brain tissue was higher in the EA group than in the control group(all P<0.01).However,the treatment group showed therapeutic effects and the above-mentioned pathological injuries caused by CUMS recovered.Conclusions EA can alleviate mitochondrial injury in rats with CUMS.

关 键 词：抑郁症 CUMS 松果菊苷 线粒体损伤 大鼠 

分 类 号：R-33[医药卫生]