白细胞介素-17介导牙髓基质金属蛋白酶表达和调节牙髓炎症的机制初探  

Mechanism of Interleukin-17 Regulating Matrix Metalloproteinase Expression and Inflammation in Dental Pulp

作　　者：刘梦余 LIU Mengyu(Department of Stomatology, Chengdu Third People's Hospital, Chengdu 610041, China)

机构地区：[1]成都市第三人民医院口腔科,四川成都610041

出　　处：《口腔医学研究》2021年第5期425-430,共6页Journal of Oral Science Research

基　　金：成都市卫健委基金项目(编号:2020002)。

摘　　要：目的:探究白细胞介素(IL)-17在牙髓炎症过程中时空表达,研究IL-17对牙髓炎可能的调节机制。方法:构建大鼠牙髓炎模型,苏木精-伊红(HE)染色观察牙髓组织结构形态,免疫组织化学染色检测牙髓中IL-17表达;培养人牙髓细胞,肿瘤细胞坏死因子(TNF)-α刺激模拟炎症状态,RT-qPCR检测IL-17基因表达,ELISA检测IL-17的蛋白表达;IL-17重组蛋白刺激牙髓细胞,RT-qPCR检测MMP-1、MMP-2、MMP-3和MMP-13的表达,胶原酶谱和明胶酶谱检测MMP-1和MMP-2酶活性;Western blot检测信号通路表达。结果:IL-17在牙髓炎症过程中持续高表达,体外TNF-α刺激可升高牙髓细胞IL-17的表达。IL-17刺激牙髓细胞,上调MMP-1和MMP-2的酶活性,并激活丝裂原蛋白激酶(MAPK)信号通路。结论:IL-17在炎症牙髓表达上调,并可通过升高MMP-1和MMP-2的表达,激活MAPK信号通路发挥促炎作用。Objective:To investigate the role of interleukin-17 in dental pulp inflammation and the relationship between IL-17 and MMPs.Methods:Immunohistochemical staining was used to detect the expression of TNF-αand IL-17 in pulp tissues.TNF-αor IL-17 recombinant protein was used to treat human dental pulp cells.RT-qPCR,Western blotting,and ELISA were used to detect the expression of mRNA and protein levels of IL-17,MMP-1,MMP-2,MMP-3 and MMP-13.Gelatin zymography and collagen zymography were used to detect the enzymatic activity of MMP-1 and MMP-2.Results:IL-17 was high-expressed during pulpitis.TNF-αtreatment could induce expression of IL-17 in HDPCs.IL-17 mainly contributed to its positive regulatory role in inflammation through up regulating MMPs and activating protein kinase MAPK pathway.Conclusion:IL-17 acts as an inflammatory mediator in dental pulp inflammation by acting MAPK pathway.IL-17 can induce the expression of MMP-1 and MMP-2 in HDPCs.

关 键 词：牙髓炎 白细胞介素-17 基质金属蛋白酶 免疫学 

分 类 号：R781.31[医药卫生—口腔医学]