Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress  

作　　者：M.Subbulakshmi Sugapriya Dhanasekaran S.Abirami M.Kannan R.Palaniappan Divya Venugopal 

机构地区：[1]Department of Microbiology,K.R.College of Arts and Science,Kovilpatti,Tamil Nadu,India [2]Department of Medical Laboratory Sciences,College of Applied Medical Sciences,Prince Sattam Bin Abdulaziz University,Wadi Al Dawaser,Saudi Arabia [3]Department of Microbiology,Kamaraj College,Thoothukudi,Tamil Nadu,India [4]Research Department of Zoology,V.H.N.Senthikumara Nadar College(Autonomus)Virudhunagar,Tamil Nadu,India [5]Research Department of Computer Science,V.H.N.Senthikumara Nadar College(Autonomus)Virudhunagar,Tamil Nadu,India

出　　处：《Food Science and Human Wellness》2021年第4期452-459,共8页食品科学与人类健康（英文）

摘　　要：Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects.This study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession number:SUB3267363).Thin layer chromatographic(TLC)profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and fl avonoid fractions with retention factor(Rf)values of 0.955 and 0.927 respectively.The GC/MS chromatograms of the SMK-1 methanolic extract identifi ed the main bioactive compound was phenol,5-methyl-2-(1-methylethyl)(74.00%)(thymol).The radical scavenging activity for the fl avonoid fraction was greater than the phenolic fractions(Rf–phenolics fractions>Rf–fl avonoid fractions)with the antioxidant activity more than that of standard ascorbic acid.Also,the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner.This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefi ts.The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.

关 键 词：Antioxidant ANTI-PROLIFERATION Dietary mushrooms HeLa cells Novel Mushrooms THYMOL 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]