DRD2在RLS大鼠模型LPAG上的变化  

The changes of dopamine D2 receptor on LPAG in RLS rat model

作　　者：张先元 陈强[2] 章正祥[2] 周会霞 邵敏峰 黄春华[5] Zhang Xianyuan

机构地区：[1]杭州市丁桥医院(杭州市中医院丁桥院区),310000 [2]浙江中医药大学附属第一医院(浙江省中医院),310000 [3]杭州市老年病医院,310000 [4]杭州市余杭区第一人民医院,310000 [5]江西中医药大学附属医院,330006

出　　处：《浙江临床医学》2021年第4期475-476,480,共3页Zhejiang Clinical Medical Journal

基　　金：国家自然科学基金资助项目(81302938);浙江省自然科学基金资助项目(LY18H270009);浙江省中医药优秀青年人才基金计划(2013ZQ015);浙江省中医药科学研究基金项目(2017ZA047,2020ZB196);浙江省医药卫生科研基金项目(2017KY118,2017KY509);2018年浙江省医药卫生科研面上项目(2018KY549)。

摘　　要：目的探索RLS大鼠模型PAG上多巴胺D2受体的变化.方法雄性SD大鼠随机分成空白组(n=3)、假手术组(n=6)、模型组(n=6).对右侧A11进行药物注射,第8天进行行为学录制和脑组织取材.对A11进行TH免疫组化染色,PAG行多巴胺D2受体免疫组化染色.结果毁损组右侧A11多巴胺神经元毁损率明显高于对侧和假手术组右侧(P<0.01).毁损组第3 h的爬笼时间高于假手术组(P<0.05).RLS模型组双侧LPAG多巴胺D2受体表达多于假手术组(P<0.01).结论毁损单侧A11后,可导致双侧LPAG的多巴胺D2受体表达增多.Objective To explore the changes of dopamine D2 receptor in the LPAG of RLS rat model.Methods Male SD rats were randomly divided into blank group(n=3),sham operation group(n=6),and model group(n=6).The right side A11 was injected with drugs,and behavioral recording and brain tissue sampling were perfonned on the 8th day.A11 was subjected to TH ininiunohistocheniical staining,and PAG was subjected to dopamine D2 receptor inimunohistochemical staining.Results(1)The damage rate of A11 dopamine neurons on the right side of the damaged group was significantly higher than that of the contralateral and sham-operated groups(P<0.01).(2)The cage climbing time at the third hour of the damage group was higher than that of the sham operation group(P<0.05).(3)The expression of bilateral LPAG dopamine D2 receptor in RLS model group was more than that in sham operation group(P<0.01).Conclusion The destruction of unilateral A11 can lead to increased expression of dopamine D2 receptors in bilateral LPAG.

关 键 词：多巴胺D2受体 RLS A11 PAG 

分 类 号：R74[医药卫生—神经病学与精神病学]