三种益生芽孢杆菌制备纳米硒能力研究  

Ability of Nano-Se Production of Three Potential Probiotic Bacillus sp.

作　　者：张鹏博 彭晴[1] 乔宇[1] 徐小轻 张宇微[1] 田丹丹 黄英 马蓝 石波[1] ZHANG Pengbo;PENG Qing;QIAO Yu;XU Xiaoqing;ZHANG Yuwei;TIAN Dandan;HUANG Ying;MA Lan;SHI Bo(Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院饲料研究所,北京100081

出　　处：《中国农业科技导报》2021年第5期176-186,共11页Journal of Agricultural Science and Technology

基　　金：国家重点研发计划项目(2018YFD0501403)。

摘　　要：纳米硒因易于被机体吸收、毒性小且具有抗肿瘤、抗氧化、增强机体免疫力等功能,而被广泛应用于各领域。为探讨不同益生芽孢杆菌制备纳米硒的能力,以阿氏芽孢杆菌SI9、解淀粉芽孢杆菌AT34和凝结芽孢杆菌LB-9为研究对象,比较其转化Na_(2)SeO_(3)合成纳米硒的能力,并对其制备的纳米硒颗粒进行形貌表征和含量测定。结果表明,阿氏芽孢杆菌SI9还原制备纳米硒能力最强,解淀粉芽孢杆菌AT34与凝结芽孢杆菌LB-9次之;随着Na_(2)SeO_(3)浓度的升高,菌株的生长均受到抑制;在Na_(2)SeO_(3)浓度为200μg·mL^(-1)时,上述三株菌的纳米硒产率分别为48.3%、36.3%、34.8%,提取率分别为96.9%、95.0%和94.0%,纯度分别为73.2%、78.1%和89.4%,对Na_(2)SeO_(3)的转化率均为100%;场发射扫描电镜观察三株菌的纳米硒颗粒尺寸均在70~500 nm之间。研究结果可为微生物法转化制备纳米硒提供理论支持。Nano-selenium is widely used in various fields because of its easy absorption,low toxicity,anti-tumor,anti-oxidation and enhancing immunity,etc.In order to study the ability of different probiotic Bacillus to prepare nano-selenium,taking Bacillus aryabhattai SI9,Bacillus amyloliquefaciens AT34 and Bacillus coagulans LB-9 as research objects,the ability of transforming Na_(2)SeO_(3)into nano-selenium was compared,and the nano-selenium particles prepared by them were characterized by morphology and content determination.The results showed that B.aryabhattai SI9 had the strongest ability to produce nano selenium,followed by B.amyloliquefaciens AT34 and B.coagulans LB-9.With the increase of Na_(2)SeO_(3)concentration,the growth of three strains was inhibited;when Na_(2)SeO_(3)concentration was 200μg·mL^(-1),the nano-selenium yields of three strains were 48.3%,36.3%,34.8%,the extraction rates were 96.9%,95.0%and 94.0%,respectively;the purity was 73.2%,78.1%and 89.4%,respectively;and the conversion rates of Na_(2)SeO_(3)were all 100%.It was showed that the sizes of selenium nanoparticles extracted by the three strains were 70~500 nm under field emission scanning electron microscopy(SEM).The present research could provide theoretical support for the preparation of nano-selenium by microbial transformation.

关 键 词：纳米硒 阿氏芽孢杆菌 解淀粉芽孢杆菌 凝结芽孢杆菌 

分 类 号：Q939.1[生物学—微生物学]