miR-7156-3p在乳腺癌中的表达及其对乳腺癌细胞增殖和迁移的影响  

Expression of miR-7156-3p in breast cancer and its effect on proliferation and migration of breast cancer cells

作　　者：刘敏 张弘[3] 程丰[1] 张茂娜 赵涓涓 LIU Min;ZHANG Hong;CHENG Feng;ZHANG Maona;ZHAO Juanjuan(Central Blood Station of Ezhou,Ezhou 436000,China;Department of Laboratory,Ezhou Central Hospital;Department of Pathology,Ezhou Central Hospital)

机构地区：[1]湖北省鄂州市中心血站,鄂州436000 [2]鄂州市中心医院检验科 [3]鄂州市中心医院病理科

出　　处：《山西医科大学学报》2021年第4期417-422,共6页Journal of Shanxi Medical University

基　　金：湖北省卫生厅科研一般项目(JX5B50)。

摘　　要：目的探讨微小RNA-7156-3p(miR-7156-3p)在乳腺癌中的表达及通过靶向调控Ras相关蛋白Rab-1A(Ras-related protein Rab-1A,RAB1A)对乳腺癌细胞增殖和迁移的影响。方法采用实时定量聚合酶链式反应(qPCR)检测miR-7156-3p在乳腺癌患者血清、人正常乳腺上皮细胞MCF10A及乳腺癌细胞株HCC1937、MCF7、MB-MDA-468、SKBR3、BT549中的表达水平。选择miR-7156-3p表达水平最少的乳腺癌细胞株,分别转染miR-NC和miR-7156-3p,标记为miR-NC组和miR-7156-3p组。分别采用四甲基偶氮唑蓝(MTT)法和Transwell小室实验检测过表达miR-7156-3p对乳腺癌细胞增殖和迁移能力的影响。通过生物信息学网站预测和双荧光素酶报告基因系统检测miR-7156-3p与靶基因的靶向关系。采用qPCR和Western blotting检测乳腺癌细胞中靶基因的表达水平。结果乳腺癌患者血清中miR-7156-3p的表达水平低于健康体检者(P<0.01)。与MCF10A细胞相比,HCC1937、MCF7、MB-MDA-468、SKBR3、BT549细胞中miR-7156-3p的表达水平均下降(P<0.05),HCC1937细胞中的变化最为明显(P<0.01)。与miR-NC组相比,miR-7156-3p组HCC1937细胞的增殖能力和迁移能力明显降低(P<0.01)。双荧光素酶报告基因系统结果证实miR-7156-3p的靶基因是RAB1A(P<0.01)。qPCR和Western blotting结果显示,miR-7156-3p负向调控RAB1A的表达(P<0.01)。结论miR-7156-3p在乳腺癌中低表达,过表达miR-7156-3p可通过靶向调控RAB1A抑制乳腺癌细胞HCC1937的增殖和迁移。Objective To investigate the expression of microRNA-7156-3p(miR-7156-3p)in breast cancer and its effect on breast cancer cell proliferation and migration by regulating Ras-related protein Rab-1A(RAB1A)expression.Methods Real-time quantitative polymerase chain reaction(qPCR)was used to detect the expression level of miR-7156-3p in serum of breast cancer patients,human normal breast epithelial cells MCF10A,and breast cancer cell lines HCC1937,MCF7,MB-MDA-468,SKBR3,BT549.The breast cancer cell line with the lowest expression level of miR-7156-3p was transfected with miR-NC and miR-7156-3p,respectively,and labeled as miR-NC group and miR-7156-3p group.The effects of miR-7156-3p overexpression on breast cancer cell proliferation and migration were detected by tetramethylazazole blue(MTT)method and Transwell chamber experiment.The target relationship between miR-7156-3p and the target gene was detected through the prediction of the bioinformatics website and the dual luciferase reporter gene system.QPCR and Western blotting were used to detect the expression level of target gene in breast cancer cells.Results The expression level of miR-7156-3p in the serum of breast cancer patients was lower than that of healthy subjects(P<0.01).Compared with MCF10A cells,the expression level of miR-7156-3p was decreased in HCC1937,MCF7,MB-MDA-468,SKBR3,BT549 cells(P<0.05),and the lowest in HCC1937 cells(P<0.01).Compared with miR-NC group,the proliferation ability and the migration ability of HCC1937 cells were inhibited in miR-7156-3p group(P<0.01).The results of the dual luciferase reporter gene system confirmed that the target gene of miR-7156-3p is RAB1A(P<0.01).The results of qPCR and Western blotting showed that miR-7156-3p negatively regulated the expression of RAB1A(P<0.01).Conclusion The expression of miR-7156-3p is low in breast cancer.Overexpression of miR-7156-3p can inhibit the proliferation and migration of breast cancer cell HCC1937 by regulating RAB1A.

关 键 词：miR-7156-3p 乳腺癌 RAB1A 细胞增殖 细胞迁移 

分 类 号：R737.9[医药卫生—肿瘤]