不同地方种群黄鳝暴露在亚硝酸钠环境中对其免疫、抗氧化和肌肉生长能力的影响  

Effect of Sodium Nitrite on Immunity,Antioxidant and Muscle Growth of Monopterus albus in Different Local Populations

作　　者：陈强[1] 吕巍巍 焦阳 黄银盈 袁泉 黄伟伟[2] 孙小琳 周文宗[2] 赵云龙[1] CHEN Qiang;LÜ Weiwei;JIAO Yang;HUANG Yinying;YUAN Quan;HUANG Weiwei;SUN Xiaolin;ZHOU Wenzong;ZHAO Yunlong(School of Life Sciences, East China Normal University, Shanghai 200241, China;Institute of Ecological Environment, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

机构地区：[1]华东师范大学生命科学学院,上海200241 [2]上海市农业科学院生态环境研究所,上海201403

出　　处：《复旦学报（自然科学版）》2021年第2期244-252,共9页Journal of Fudan University：Natural Science

基　　金：上海市农业委员会科技兴农推广项目(沪农科推字(2018)第4-7号)。

摘　　要：亚硝酸盐是自然界和人工养殖环境中常见的含氮化合物,对水产养殖动物具有毒害作用.本文利用亚硝酸钠对江西鄱阳湖(PYH)、湖南洞庭湖(DTH)、安徽巢湖(CH)、江苏洪泽湖(HZH)和太湖(TH)野生黄鳝(Monopterus albus)种群进行暴露,检测其肝脏组织中MHC-2A、Hepcidin、COX、5-PO和HSP等免疫炎症相关基因的表达水平,肝脏组织抗氧化系统中的SOD和CAT酶活性、GSH和GSSG及氧化应激标志LPO和MDA含量,以及肌肉中MYF5、MYOD1、MRF4和MYOG等生肌调节因子相关基因的表达水平.结果表明不同地方黄鳝种群暴露在亚硝酸盐环境中,PYH群体和DTH群体的免疫能力相对更高;PYH、CH和HZH群体炎症反应较轻;早期暴露中PYH群体和CH群体抗氧化能力较强;CH群体具有更好的肌肉生长分化能力.说明不同地理黄鳝群体在环境压力下生理功能存在差异,可为人工选育抗压性强的黄鳝品系提供技术支持.Nitrite is a common nitrogen-containing compound in nature and artificial culture environment,and has a toxic effect on aquaculture animals.We used sodium nitrite to expose wild rice eel Monopterus albus populations in five lakes(Poyang lake,Dongting lake,Hongze lake,Caohu lake,Taihu lake)to detect the expression levels of immune and inflammatory related genes such as MHC-2A,Hepcidin,COX,5-PO and HSP in the liver,we also detected the SOD and CAT enzymes activity,GSH and GSSG content and oxidative stress markers LPO and MDA content in the antioxidant system of liver.Furthermore,genes related to myogenic regulatory factors in muscle,MYOG,MYOD,MRF4,and MYF5,have also been tested for their expression levels.The results showed that the immune capacity of PYH and DTH populations of rice eel in different local populations was relatively higher when exposed to nitrite;PYH,CH and HZH populations had less inflammation;PYH and CH populations had high anti-oxidation ability during early exposure;CH population has better muscle growth and differentiation ability.Our results prove that there are differences in the physiological functions of rice eels from different geographical groups when facing the environmental stress,which provides a potential for selective genetic cross breeding and lays a foundation for the artificial selection of excellent varieties of rice field eels.

关 键 词：黄鳝 地理种群 亚硝酸盐 抗氧化 免疫 生肌调节因子 

分 类 号：Q175[生物学—水生生物学]