液相色谱-质谱/质谱法测定小甑酒中甜蜜素含量的不确定度评定  

Uncertainty Assessment for the Determination of Sweetener in Xiaozhen Wine by Liquid Chromatography-mass Spectrometry/Mass Spectrometry

作　　者：方岚 方磊 丁野 丁盈 戴慧 FANG Lan;FANG Lei;DING Ye;DING Ying;DAI Hui(Yunnan Food and Drug Audit and Inspection Center,Kunming 650106,China;Hunan Institute for Drug Control(Hunan Center for Pharmaceutical Excipients Control),Changsha 410001,China;Hunan Pharmaceutical Quality Evaluation Engineering Technology Research Center,Changsha 410001,China;Hunan Food and Drug Vocational College,Changsha 410208;Hunan University of Chinese Medicine,Changsha 410036,China)

机构地区：[1]云南省食品药品审核查验中心,云南昆明650106 [2]湖南省药品检验研究院(湖南药用辅料检验检测中心),湖南长沙410001 [3]湖南省药品质量评价工程技术研究中心,湖南长沙410001 [4]湖南省食品药品职业学院,湖南长沙410208 [5]湖南中医药大学,湖南长沙410036

出　　处：《云南师范大学学报（自然科学版）》2021年第3期57-62,共6页Journal of Yunnan Normal University:Natural Sciences Edition

摘　　要：按照SN/T 1948-2007《进出口食品中环己基氨基磺酸钠的检测方法》,采用液相色谱-质谱/质谱法对小甄酒中甜蜜素含量进行测定;依据CNAS-GL006:2019《化学分析中不确定度的评估指南》建立数学模型,对影响测量结果的不确定度来源如标准曲线的配制、样品的称量、样品的制备、标准曲线的拟合以及样品测量重复性等因素进行了分析和评定.小甄酒中甜蜜素的含量为0.000348 g/kg时,其含量的扩展不确定度为1.065×10^(-5)g/kg,测定结果不确定可报告为(0.000348±1.065×10^(-5))g/kg(P=95%,k=2).The content of sweetener in Xiaozhen wine was determined by liquid chromatography-mass spectrometry/mass spectrometry according to SN/T 1948-2007"Determination method of sodium cyclohexylaminosulfonate in imported and exported foods";A mathematical model was established according to CNAS-GL006:2019"Guidelines for the assessment of uncertainty in chemical analysis",and the sources of uncertainty affecting the measurement results,such as the preparation of the standard curve,sample weighing,sample preparation,standard curve fitting,sample measurement repeatability,and other factors were analyzed and evaluated.The extended uncertainty of the content of sweetener in Xiaozhen wine was 1.065×10^(-5)g/kg at 0.000348 g/kg,and the uncertainty of the measurement result could be reported as(0.000348±1.065×10^(-5))g/kg(P=95%,k=2).

关 键 词：小甄酒 甜蜜素 不确定度 液相色谱-质谱/质谱法 

分 类 号：O65[理学—分析化学]