两种胃癌细胞株与小鼠原代脾脏淋巴细胞共培养对Th17细胞分化的影响  

Effects of co-culture of two gastric cancer cell lines and mouse primary splenic lymphocytes on Th17 cell differentiation

作　　者：刘畅[1] 陈阳 叶佳 朱梅萍[1] LIU Chang;CHEN Yang;YE Jia;ZHU Mei-ping(Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,CHINA;Department of Emergency Medicine,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,CHINA)

机构地区：[1]上海中医药大学附属曙光医院消化科,上海201203 [2]上海中医药大学附属岳阳中西医结合医院急诊内科,上海200437

出　　处：《海南医学》2021年第9期1089-1091,共3页Hainan Medical Journal

基　　金：上海中医药大学附属曙光医院四明青年基金项目(编号:SGKJ-201801);上海市科学技术委员会“2017年度医学引导类(中、西医)科技支撑”项目(编号:17401935100);首届全国名中医传承工作室建设项目(编号:MZYGZS-2017003)。

摘　　要：目的观察不同胃癌细胞株与CD4^(+)T细胞共培养后Th17细胞分化的改变情况。方法将分离并鉴定后的小鼠原代脾脏淋巴细胞分别与人胃癌SGC-7901细胞株、人胃癌BGC-823细胞株共培养,分为SGC-7901共培养组、BGC-823共培养组和对照组。以细胞内细胞因子染色FCM测定Th17细胞,计算CD4^(+)IL-17A^(+)细胞数量百分比。结果SGC-7901共培养组、BGC-823共培养组、对照组的CD4^(+)IL-17A^(+)细胞数量分别为(3.97±0.27)%、(5.17±0.14)%、(1.96±0.29)%,与SGC-7901共培养组比较,BGC-823共培养组Th17细胞数量均显著增多,差异有统计学意义(P<0.05)。结论在胃癌环境下,CD4^(+)T细胞向Th17细胞分化增多。Objective To observe the differentiation of Th17 cells in different gastric cancer cell lines co-cultured with CD4^(+)T cells.Methods The isolated and identified mouse spleen lymphocytes were co-cultured with human gastric cancer SGC-7901 cell line and human gastric cancer BGC-823 cell line,and divided into SGC-7901 co-culture group,BGC-823 co-culture group,and control group.Th17 cells were detected by FCM and the percentage of CD4^(+)IL-17A^(+)cells was calculated.Results The number(%)of CD4^(+)IL-17A^(+)cells in SGC-7901 co-culture group,BGC-823 co-culture group,and control group were(3.97±0.27)%,(5.17±0.14)%,(1.96±0.29)%.Compared with SGC-7901 co-culture group,the number of Th17 cells in BGC-823 co-culture group was significantly increased(P<0.05).Conclusion The differentiation of CD4^(+)T cells into Th17 cells is increased in gastric cancer environment.

关 键 词：小鼠原代脾脏淋巴细胞 人胃癌SGC-7901细胞株 人胃癌BGC-823细胞株 CD4^(+)T细胞 TH17细胞 

分 类 号：R-332[医药卫生]