凝胶渗透色谱-高效液相色谱法测定火麻仁油中Δ9-四氢大麻酚含量  

Determination of Δ-tetrahydrocannabinol in hempseed oils by gel permeation chromatograph-high performance liquid chromatography method

作　　者：吴丽琳 唐庆强 陈迪 张莹 杨方 WU Lilin;TANG Qingqiang;CHEN Di;ZHANG Ying;YANG Fang(Sanming Customs,Fujian Sanming 365000,China;Technology Center of Fuzhou Customs District,Fujian Fuzhou 350001,China;Agricultural and Rural Bureau of Ninghua,Fujian Sanming 365000,China)

机构地区：[1]三明海关,福建三明365000 [2]福州海关技术中心,福建福州350001 [3]宁化县农业农村局,福建三明365000

出　　处：《中国食品卫生杂志》2021年第1期35-39,共5页Chinese Journal of Food Hygiene

基　　金：海关总署科研项目(2020HK188);福建省自然基金项目(2020J1098);福州市科技计划项目(2020-S-34)。

摘　　要：目的建立使用凝胶渗透色谱(GPC)净化,高效液相色谱(HPLC)分析测定火麻仁油中Δ^(9)-四氢大麻酚(Δ^(9)-THC)的检测方法。方法样品经环已烷和乙酸乙酯溶解,经GPC净化分离,再以Agilent Eclipse XDB-C18色谱柱(150 mm×4.6 mm, 3μm)分离,以乙腈-水流动相洗脱,二级管阵列检测器分析,外标法定量。结果该方法在0~500μg/L浓度内,线性相关系数(r)>0.999,方法检测限(LOD)为0.05 mg/kg,定量限(LOQ)为0.17 mg/kg。加标回收率在84.6%~101.8%之间,相对标准偏差为3.2%~4.7%(n=6)。在实际样品检测中,10种不同品牌的火麻仁油中Δ^(9)-THC含量在0.40~5.82 mg/kg之间。结论该方法稳定性好,灵敏度高,适用于火麻仁油样品中Δ9-THC的检测分析。Objective A method for determination of Δ9-tetrahydrocannabinol(Δ9-THC) in hempseed oil by gel permeation chromatograph-high performance liquid chromatography(GPC-HPLC) method was developed. Methods Samples were extracted with cyclohexane and ethyl acetate and the extract was purified by GPC,then separated by Agilent Eclipse XDB-C18 column(150 mm×4.6 mm, 3 μm).The acetonitrile-water was used as mobile phase, and quantified by secondary tube array detector and external standard method. Results The linear correlation coefficient(r) of this method was above 0.999 in the concentration range of 0-500 μg/L.Δ9-THC had a limit of detection(LOD) of 0.05 mg/kg and the limit of quantity(LOQ) of 0.17 mg/kg. Recoveries were between 84.6% and 101.8% and the relative standard deviation were within 3.2%-4.7%(n=6). With the actual samples tested, Δ9-THC content in 10 different brands of hempseed oil ranges from 0.40-5.82 mg/kg. Conclusion The results showed that the method had good stability and high sensitivity, and was suitable for determination of Δ9-THC in hempseed oil.

关 键 词：大麻素 Δ9-四氢大麻酚 火麻仁油 凝胶渗透色谱 高效液相色谱 

分 类 号：R155[医药卫生—营养与食品卫生学]