原花青素对糖尿病牙周炎大鼠相关炎性因子的影响  

Effect of Proanthocyanidins on Related Inflammatory Factors in Diabetic Rats with Periodontitis

作　　者：崔灵欣 仵楠[1] 徐江[1] 朱晓娟 李艳[2] 代海涛[1] CUI Ling-xin;WU Nan;XU Jiang;ZHU Xiao-juan;LI Yan;DAI Hai-tao(Department of Stomatology,the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi,832008;Shihezi University School of Medicine,Xinjiang Shihezi,832002)

机构地区：[1]石河子大学医学院第一附属医院口腔科,新疆石河子832008 [2]石河子大学医学院,新疆石河子832002

出　　处：《农垦医学》2021年第1期6-10,共5页Journal of Nongken Medicine

基　　金：自治区研究生教育教学改革项目(XJ2020G01);石河子大学科研课题(ZZZC201819A)。

摘　　要：目的:通过检测糖尿病牙周炎大鼠牙周组织中相关炎性因子的表达,探究葡萄籽原花青素提取物(GSPE)对糖尿病牙周炎是否存在影响。方法:选择健康雄性SD大鼠45只随机分为三组:正常对照组(H)、糖尿病牙周炎组(P)和糖尿病牙周炎+GSPE治疗组(G)。采用牙周结扎+高糖高脂饮食+腹腔注射链脲佐菌素建立糖尿病牙周炎大鼠模型,建模后G组给予浓度为100mg/kg GSPE溶液连续灌胃给药40天;检查牙周探诊深度(PD)及附着水平(AL),HE染色观察牙周组织形态,免疫组化染色检测上颌第二磨牙牙龈组织中TNF-α和IL-1β的表达水平。结果:相较H组,P组大鼠牙周组织炎症显著,PD、AL均明显增加,差异具有统计学意义(P<0.05),牙龈组织中TNF-α、IL-1β表达均明显升高,差异具有统计学意义(P<0.05);而G组牙周炎症好转,PD、AL均低于P组,差异具有统计学意义(P<0.05),同时牙龈组织中TNF-α、IL-1β的表达均低于P组,差异具有统计学意义(P<0.05)。结论:GSPE能抑制糖尿病牙周炎大鼠牙龈组织中TNF-α、IL-1β表达,改善其牙周炎症状态及牙槽骨破坏程度,对糖尿病牙周炎具有一定辅助治疗作用。Objective:To investigate the effect of grape seed proanthocyanidin extract(GSPE)on diabetic periodontitis,we detected the expression of related inflammatory factors in the periodontal tissues of diabetic periodontitis rats.Methods:Forty-five male Sprague-Dawley rats were randomly assigned to different groups as follows:control(H),DP(P)and DP+GSPE group(G).The DP model was established by local ligation method+high-fat diet+intraperitoneal injection of STZ.After successful modeling,group G was given GSPE with a concentration of100 mg/kg for continuous gavage administration for 40 days.Hematoxylin-Eosin staining,the depth of periodontal probe,attachment loss,and immunohistochemical staining were performed.Results:Compared with group H,periodontal tissue inflammation was significantly increased in group P,and both PD and AL were significantly increased,the differences were statistically significant(P<0.05),the expression of TNF-αand IL-1βin gingival tissue was significantly increased,and the difference was statistically significant(P<0.05);However,periodontitis improved in group G,PD and AL were lower than those in group P,and the differences were statistically significant(P<0.05),and the expression of TNF-αand IL-1βin gingival tissue was lower than that in P group,and the difference was statistically significant(P<0.05).Conclusion:GSPE can inhibit the expression of TNF-αand IL-1βin gingival tissue of rats with diabetic periodontitis,improve the periodontitis state and the degree of alveolar bone destruction,and have a certain adjuvant effect on diabetic periodontitis.

关 键 词：葡萄籽原花青素提取物 糖尿病牙周炎 TNF-Α IL-1Β 

分 类 号：R780.2[医药卫生—口腔医学]