基于效应子E38识别与NB-LRR富集测序挖掘抗晚疫病马铃薯资源  

Identification of Potato Resistance Resource Through Phytophthora infestans Effector E38 Recognition and NB-LRR Enrichment Sequencing

作　　者：段艳凤[1] 文国宏 Armstrong M R 李广存[1] 金黎平[1] DUAN Yanfeng;WEN Guohong;Armstrong M R;LI Guangcun;JIN Liping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Potato Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070,China;The University of Dundee,Division of Plant Sciences at the James Hutton Institute,Dundee,DD25DA,UK)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]甘肃省农业科学院马铃薯研究所,甘肃兰州730070 [3]英国邓迪大学/詹姆斯赫顿研究所植物科学系,英国邓迪DD25DA

出　　处：《中国马铃薯》2021年第2期97-105,共9页Chinese Potato Journal

基　　金：现代农业产业技术体系专项资金资助(CARS-9)。

摘　　要：为挖掘抗晚疫病马铃薯资源,研究利用4个不同毒力的晚疫病菌株,基于离体叶片接种法对四倍体材料‘陇薯12号’和‘L05Nsr-j-1’进行抗性鉴定。结果表明‘陇薯12号’高抗除"超级生理小种"CN152外的其余3个菌株,‘L05Nsr-j-1’则对4个菌株均表现为高感;进一步利用晚疫病菌效应子E38在上述2份材料上进行农杆菌介导的瞬时表达,发现只有‘陇薯12号’可以识别E38产生特异的HR反应;抗病基因富集测序诊断(dRenSeq)结果表明‘陇薯12号’不含已知的晚疫病抗病基因,预示‘陇薯12号’含有未知的抗病基因;利用E38对‘陇薯12号’和‘L05Nsr-j-1’的F1群体215个单株进行农杆菌介导的瞬时表达检测,结果显示67%的单株出现假阳性反应,表明其F1群体不适合利用农杆菌介导的效应子识别鉴别基因型。研究结果为今后‘陇薯12号’的利用及其晚疫病抗性基因的克隆奠定了基础。Four Phytophthora infestans isolates with different virulence were used to evaluate late blight resistance on tetraploid potatoes,'Longshu 12'and'L05Nsr-j-1',by detached leave assay in order to mine the potato resistance resources.The results showed that'Longshu 12'was high resistant to other three isolates with exception of'super-isolate'CN152,while'L05Nsr-j-1'was high sensitive to all the four isolates.P.infestans effector E38 was further used to identify the above two materials by Agrobacterium-mediated transient expression,and it was found that only'Longshu 12'could recognize E38 to induce a specific hypersensitive cell death.Diagnostic resistance gene enrichment(dRenSeq)revealed that'Longshu 12'did not contain previously identified nucleotide-binding,leucine-rich repeat late blight resistance genes,suggesting that'Longshu 12'contains potential resistance gene(s),and it might be a distinct new R gene(s).The E38 was used to recognize 215 F1 population genotypes derived from'Longshu 12'and'L05Nsr-j-1',and the results showed that 67%of the genotypes had a false positive reaction,indicating that F_(1)population derived from'Longshu 12'and'L05Nsr-j-1'was not suitable for high-throughput effectoromics screens that were typically based on Agrobacterium-based effector recognition studies.The results of this study would lay the foundation for the future use of'Longshu 12'and the cloning of late blight resistance genes.

关 键 词：马铃薯 晚疫病 效应子 抗病基因 

分 类 号：S532[农业科学—作物学]