马铃薯脯氨酸转运体StProT3的序列结构及表达分析  

Sequence Structure and Expression Analysis of Potato Proline Transporter StProT3

作　　者：王明[1] 王万兴[2] 何长征[1] 胡新喜[1] 熊兴耀[2] 秦玉芝[1] WANG Ming;WANG Wanxing;HE Changzheng;HU Xinxi;XIONG Xingyao;QIN Yuzhi(College of Horticulture and Landscape,Hunan Agricultural University,Changsha,Hunan 410128,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国马铃薯》2021年第2期118-126,共9页Chinese Potato Journal

基　　金：国家自然科学基金项目(31371683)。

摘　　要：依据马铃薯转录组数据,鉴定马铃薯脯氨酸转运体(Proline transporter,ProTs)在逆境中的作用。以马铃薯GS393(Solanum commersonii-LZ3.4-Wisconsin,United States)为材料,采用实时荧光定量PCR对StProT3基因在不同组织及激素、重金属、盐、干旱和低温处理下的表达模式进行分析。该基因CDS长度为1317 bp,编码438个氨基酸。序列比对显示与SlyProT3有96%的相似性,因而命名为StProT3(NCBI登陆号为MH027990.1)。StProT3基因在马铃薯根、茎、叶、匍匐茎以及块茎中均有表达,其中根中表达量最高;重金属(氯化汞、氯化镉、氯化铜、氯化铝)、干旱、盐渍、激素(IAA、GA3、6-BA)处理能诱导该基因表达,而低温和ABA处理则抑制该基因表达,这表明StProT3基因参与了马铃薯的激素信号传导以及非生物胁迫响应。GS393(Solanum commersonii-LZ3.4-Wisconsin,United States)was used as the material,and the expression pattern of StProT3 gene in different tissues and under the treatments of hormones,heavy metals,salt,drought and low temperature was detected by real-time fluorescent quantitative PCR in order to understand the role of potato proline transporter(ProTs)in stress,based on potato transcriptome data.The CDS of this gene was 1317 bp in length and encoded 438 amino acids.The sequence alignment showed 96%similarity to SlyProT3 and was named StProT3(NCBI accession number MH027990.1).The StProT3 gene was expressed in potato roots,stems,leaves,stolons and tubers,with the highest expression in roots.Heavy metals(mercury chloride,cadmium chloride,copper chloride,and aluminum chloride),drought,salt,hormone treatment with(IAA,GA3 and 6-BA)induced expression of the gene.However,low temperature and ABA treatment inhibited expression of the gene.The StProT3 gene is involved in the hormone signaling and abiotic stress response of potato.

关 键 词：马铃薯 脯氨酸转运体 实时荧光定量 非生物胁迫 

分 类 号：S532[农业科学—作物学]