甲状腺乳头状癌中PBX3和Twist表达的临床意义  

The expression of PBX3 and Twist in papillary thyroid carcinoma and its clinical significance

作　　者：曹学全 蔡小波 杨朝晖 魏科娜 阮正英 周璐青 卢洪胜 CAO Xuequan;Cai Xiaobo;YANG Zhaohui;WEI Kena;RUAN Zhengying;ZHOU Luqing;LU Hongsheng(Department of Pathology,Taizhou Central Hospital(Affiliated Hospital to Taizhou College),Taizhou 318000,China)

机构地区：[1]台州市中心医院(台州学院附属医院)病理科,浙江台州318000

出　　处：《温州医科大学学报》2021年第5期358-363,共6页Journal of Wenzhou Medical University

基　　金：台州市科技计划项目(1901ky44);浙江省医药卫生科技计划项目(2021KY1219)。

摘　　要：目的:研究前B细胞白血病同源盒基因3(PBX3)和Twist在甲状腺乳头状癌(PTC)中m RNA和蛋白的表达特点,探讨其表达的临床意义。方法:收集60例PTC和30例相应癌旁组织(距离肿瘤≥2 cm)的手术切除标本,应用免疫组化En Vision二步法检测石蜡包埋组织PBX3和Twist蛋白的表达,实时荧光定量PCR检测新鲜组织中PBX3和Twist m RNA的表达水平,通过统计学软件分析PTC中PBX3和Twist的m RNA和蛋白表达与患者不同临床病理特征的关系,并对二者蛋白表达的相关性采用Spearman等级相关分析。结果:实时荧光定量PCR结果显示,PTC组织中PBX3和Twist mRNA表达水平均高于癌旁组织(P<0.05);免疫组化结果显示,PTC组织中PBX3和Twist蛋白的阳性表达率分别为73.33%(44/60)和68.33%(41/60),癌旁组织分别为16.67%(5/30)和13.33%(4/30),癌组织中的表达显著高于癌旁组织(P<0.05)。伴有包膜侵犯、淋巴结发生转移和Ⅲ~Ⅳ期PTC组织中PBX3 mRNA和蛋白表达均高于无包膜侵犯、无淋巴结转移及Ⅰ~Ⅱ期PTC组织(P<0.05)。包膜是否侵犯、淋巴结是否转移及Ⅰ~Ⅱ期和Ⅲ~Ⅳ期PTC组织中Twist mRNA和蛋白表达均有差异(P<0.05)。PBX3和Twist蛋白在PTC中的表达呈明显的正相关(r=0.400,P<0.05)。结论:PTC组织中PBX3和Twist m RNA和蛋白表达均出现上调,其异常高表达与PTC的发生、侵袭和转移密切相关。PBX3和Twist的表达呈正相关,表明二者表达变化可能在PTC的进展中起协同作用。Objective: To study the expression of(PBX3) and Twist mRNA and protein in thyroid papillary carcinoma(PTC), and to explore their clinical significance. Methods: The expression of PBX3 and twist in paraffin embedded tissues was detected by immunohistochemical En Vision two-step method, and the expression of PBX3 and Twist mRNA in fresh tissues was detected by real-time fluorescence quantitative PCR. The expression of PBX3 and Twist mRNA and protein in PTC and its relationship with different clinicopathological characteristics and was analyzed by statistical software. The relationship between the expression of PBX3 and Twist protein was analyzed by Spearman rank correlation analysis. Results: The expression level of PBX3 and Twist mRNA in PTC was higher than that in paracancerous tissue(P<0.05), and the expression rate of PBX3 and Twist protein in PTC was 73.33%(44/60) and 68.33%(41/60), respectively. The expression of PBX3 and Twist protein in cancer tissues was significantly higher than that in adjacent tissues. The expression rate of PBX3 and Twist protein in paracancerous tissue was 16.67%(5/30) and 13.33%(4/30), respectively(P<0.05). The expression of PBX3 m RNA and protein in PTC tissues with capsule invasion, lymph node metastasis and stage Ⅲ-Ⅳ were higher than those in PTC tissues without capsule invasion, lymph node metastasis and stage Ⅰ-Ⅱ(P<0.05). The expression of Twist mRNA and protein in PTC tissues was different(P<0.05) in the presence or absence of capsule invasion, lymph node metastasis, and at stage Ⅰ to Ⅱ or stage Ⅲ to Ⅳ. There was a significantly positive correlation between PBX3 and Twist protein expression in PTC(r=0.400, P<0.05). Conclusion: The expression of PBX3 and Twist mRNA and protein is up-regulated in PTC tissue. The abnormal high expression of PBX3 and Twist is closely related to the occurrence, invasion and metastasis of PTC. The expression of PBX3 is positively correlated to the expression of Twist, indicating that the expression of PBX3 and Twist may play a synergi

关 键 词：甲状腺乳头状癌 前B细胞白血病同源盒基因3 TWIST 实时荧光定量PCR 

分 类 号：R73-3[医药卫生—肿瘤]