基于基因组高通量测序的脑胶质瘤组织中差异表达长链非编码RNA筛选及验证  

Screening and identification of lncRNA in glioma tissues based on high-throughput sequencing

作　　者：高元峰 王瑞颖 易柳 李珊[1] 肖望重[1] GAO Yuanfeng;WANG Ruiying;YI Liu;LI Shan;XIAO Wangzhong(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南中医药大学

出　　处：《山东医药》2021年第14期24-27,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81803641);湖南省自然基金面上项目(2020JJ4069);长沙科技局科技计划项目(kq1706060)。

摘　　要：目的基于基因组高通量测序筛选脑胶质瘤组织中异常表达的长链非编码RNA(lncRNA),并验证其结果。方法收集19例脑胶质瘤组织和5例正常脑组织,进行基因组高通量测序,应用edgeR软件进行两样本间差异基因分析,计算两样本间的差异倍数和P值,筛选出差异表达的lncRNA。另收集70例脑胶质瘤组织和20例正常脑组织,采用qPCR法验证脑胶质瘤组织中表达上调和下调前3位lncRNA的表达水平。结果脑胶质瘤组织中存在明显的差异表达的lncRNA共3040个,其中表达上调1233个、下调1807个。qPCR结果显示,差异表达上调前3位的TCONS_00010961、ENST00000443546.1、ENSG00000262119.1表达水平较正常脑组织升高,而差异表达下调前3位的ENST00000531508.1、TCONS_00004034、ENST00000504876.1表达水平较正常脑组织下降(P<0.05或<0.01),与基因组高通量测序结果一致。结论从脑胶质瘤组织中筛选出差异表达的lncRNA,经验证与测序结果一致;为其用于胶质瘤的临床诊断、治疗、预后提供理论依据及奠定前期的研究基础。Objective To screen out the differentially expressed long non-coding RNA(LncRNA)in the glioma tis⁃sues and to verify the results.Methods We collected 19 cases of glioma tissues and 5 cases of normal brain tissues and conducted the genome high-throughput sequencing.EdgeR software was used to analyze the expression profiles of lncRNA,and the differentially expressed lncRNAs were screened out based on fold change and P-value.In addition,70 cases of glio⁃ma tissues and 20 cases of normal brain tissues were collected,and qPCR was used to verify the first 3 up-regulated and down-regulated lncRNAs in the glioma tissues.Results There were 3040 differentially expressed lncRNAs in the glioma tissues,among which,1233 up-regulated and 1807 down-regulated lncRNAs were detected.TCONS_00010961,ENST00000443546.1,and ENSG00000262119 were the first 3 up-regulated lncRNAs whose expression was higher than that of the normal brain tissues;and ENST00000531508.1,TCONS_00004034,and ENST00000504876.1 were the first 3 down-regulated lncRNAs whose expression was lower than that of the normal brain tissues,and the outcomes were consis⁃tent with the sequencing results.Conclusions The differentially expressed lncRNAs are screened out in the glioma tis⁃sues.This founding lays the foundation for further studies,and provides theoretical basis for the clinical diagnosis,treat⁃ment and prognosis of glioma.

关 键 词：脑胶质瘤 长链非编码RNA 基因组高通量测序 差异表达基因 

分 类 号：R739.4[医药卫生—肿瘤]