食源与人源性沙门氏菌的血清和耐药水平差异分析  

Analysis of serum and drug resistance levels between food sourceand human salmonella

作　　者：毛旭建 屠博文[1] 薛银刚 赵莹 黎俊宏[1] 杜强[1] MAO Xujian;TU Bowen;XUE Yingang;ZHAO Ying;LI Junhong;DU Qiang(Changzhou Center for Disease Control and Prevention,Changzhou,Jiangsu 213022,China;School of Environmental and Safety Engineering,Changzhou University,Changzhou,Jiangsu 213003,China)

机构地区：[1]江苏省常州市疾病预防控制中心检测检验中心,江苏常州213022 [2]常州大学环境与安全工程学院

出　　处：《公共卫生与预防医学》2021年第3期63-67,共5页Journal of Public Health and Preventive Medicine

基　　金：常州市卫生健康青苗人才培养工程资助(CZQM2020113);江苏省预防医学会基金(Y2018023);江苏省卫生健康委科研课题面上项目(H2018098);常州市重大科技支撑计划(社会发展)(CE20185048及CE20175022);江苏省预防医学会课题(Y2018023);常州市应用基础研究计划(CJ20200105)。

摘　　要：目的分析2012—2018年常州市食源性沙门氏菌和人源沙门氏菌分离株的血清型分布、耐药情况、喹诺酮耐药基因携带状况和菌株亲缘关系,为沙门氏菌的防治提供科学依据。方法利用血清凝集和液相芯片鉴定血清型,采用微量肉汤稀释法测定抗生素敏感性,基因序列测定法确定喹诺酮类抗生素耐药基因,对耐喹诺酮的沙门氏菌进行多位点序列(multilocus sequence typing,MLST)分型,并用BioNumerics 8.0分析亲缘关系。结果46株食源性沙门氏菌和152株人源沙门氏菌分别检测出10种和36种血清型,优势血清型分别为印第安纳沙门氏菌和鼠伤寒沙门氏菌;两种来源沙门氏菌都以红霉素耐药率最高,多重耐药菌分别占比为93.47%(43/46)和80.92%(123/152);38株喹诺酮耐药的食源性沙门氏菌GyrA亚基主要发生双突变Asp87Asn、Ser83Phe,ParC亚基主要发生单突变Ser80Arg,119株喹诺酮耐药的人源沙门氏菌qnrS基因检出率较高,达到了68.1%(81/119);两种来源的耐喹诺酮沙门氏菌的优势ST型分别为ST17和ST19。结论常州市两种来源沙门氏菌耐药菌抗生素敏感性一致;具有协同耐药的情况,但是两者喹诺酮耐药基因突变和携带又不一致;耐喹诺酮菌株ST型分布也不一致,亲缘关系较远。提示我们本地区通过食物传播引起沙门氏菌耐药菌感染的概率较小,两者的治疗应区别用药。Objective To analyze serotype distribution,drug resistance,quinolone resistance gene carrying status and genetic relationshiPof foodborne Salmonella and human Salmonella isolates in Changzhou from 2012 to 2018,to provide scientific basis for the prevention and control of Salmonella.Methods The serum type was identified by serum agglutination and liquid chip.The antibiotic sensitivity was determined by micro broth dilution method.The quinolone antibiotic resistance gene was determined by gene sequencing method.The multilocus sequence typing(MLST)typing was performed on quinolone-resistant Salmonella,and the genetic relationship was analyzed by BioNumerics 8.0.Results A total of 10 and 36 serotypes were detected in 46 foodborne Salmonella strains and 152 human Salmonella strains,respectively.The dominant serotypes were Indiana Salmonella and Salmonella typhimurium.Erythromycin resistance rate was the highest in both Salmonella strains,and the proportion of multidrug-resistant bacteria was 93.47%(43/46)and 80.92%(123/152),respectively.38 strains of quinolone-resistant foodborne Salmonella GyrA subunit mainly occurred double mutations Asp87Asn,Ser83Phe,ParC subunit mainly occurred single mutation Ser80Arg,119 strains of quinolone-resistant human Salmonella qnrS gene detection rate was higher,reached 68.1%(81/119);The dominant ST types of quinolone-resistant Salmonella from two sources were ST17 and ST19,respectively.Conclusions The antibiotic sensitivity of the two Salmonella resistant strains from Changzhou was the same;Synergistic drug resistance,but both quinolone resistance genemutations and carry inconsistent;The ST type distribution of quinolone resistant strains isalso inconsistent,and the genetic relationship is far.It is suggested that the probability of Salmonella resistant bacteria infection caused by food transmission in our region is small,and the treatment of the two should be differentiated.

关 键 词：食源性沙门氏菌 人源沙门氏菌 血清型 喹诺酮类耐药基因 多位点序列分型 

分 类 号：R155[医药卫生—营养与食品卫生学]