miR-577通过靶向调控HOXA1对乳腺癌MDB-MA-231细胞增殖、侵袭和迁移的影响  

Effects of miR-577 Inhibits Proliferation,Migration and Invasion of Breast Cancer MDB-MA-231 Cells by Targeting HOXA1

作　　者：丁保锋 DING Baofeng(The Central Hospital of Jiamusi City,Jiamusi 154002,China)

机构地区：[1]黑龙江佳木斯市中心医院,黑龙江佳木斯154002

出　　处：《中国医学创新》2021年第10期13-18,共6页Medical Innovation of China

摘　　要：目的:探讨miR-577靶向调控HOXA1对乳腺癌MDB-MA-231细胞增殖、侵袭和迁移的影响。方法:将体外培养的MDB-MA-231细胞分为对照组(未处理)、miR-NC组(转染miR-577模拟物阴性对照)、miR-577组(转染miR-577模拟物)、miR-577+pcDNA组(转染miR-577模拟物和pcDNA3.1质粒)和miR-577+HOXA1组(转染miR-577模拟物和pcDNA3.1-HOXA1质粒),采用实时荧光定量PCR测定MDB-MA-231细胞中miR-577和HOXA1 mRNA的表达,Western blot测定HOXA1蛋白表达,荧光素酶报告基因实验测定miR-577和HOXA1的靶向关系,CCK-8法测定MDB-MA-231细胞增殖,流式细胞仪测定细胞周期分布,Transwell小室测定MDB-MA-231细胞侵袭和迁移。结果:与对照组、miR-NC组相比,miR-577组细胞中miR-577表达水平、G0/G1期细胞百分比升高,HOXA1 mRNA和蛋白表达水平、48与72 h细胞增殖活力、S期细胞百分比、侵袭细胞数和迁移细胞数均降低,差异均有统计学意义(P<0.05)。与miR-577组、miR-577+pcDNA组相比,miR-577+HOXA1组中HOXA1 mRNA和蛋白表达水平、48与72 h细胞增殖活力、S期细胞百分比、侵袭细胞数和迁移细胞数均升高,G0/G1期百分比降低,差异均有统计学意义(P<0.05)。结论:miR-577可通过靶向HOXA1抑制MDB-MA-231细胞增殖、侵袭和迁移。Objective:To investigate the effects of miR-577 targeting HOXA1 on proliferation,invasion and migration of breast cancer MDB-MA-231 cells.Method:MDB-MA-231 cells cultured in vitro were divided into control group(untreated),miR-NC group(transfected negative control of miR-577 mimics),miR-577 group(transfected miR-577mimics),miR-577+pcDNA group(transfected miR-577 mimics and pcDNA 3.1 plasmids)and miR-577+HOXA1 group(transfected miR-577 mimics and pcDNA3.1-HOXA1 plasmids).The expression of miR-577 and HOXA1 mRNA in MDB-MA-231 cells were detected by real-time fluorescence quantitative PCR,the expression of HOXA1 protein was measured by Western blot,the targeting relationship between miR-577 and HOXA1 was checked by double luciferase reporter gene assay,the proliferation of MDB-MA-231 cells was tested by CCK-8,the cell cycle was examed by flow cytometry,the migration and invasion of MDB-MA-231 cells were detected by Transwell chamber.Result:Compared with the control group and the miR-NC group,the expression level of miR-577 and the percentage of G0/G1 phase cells in the miR-577 group were increased,the expression levels of HOXA1 mRNA and protein,the proliferation activity of cells at 48 and 72 h,the percentage of cells in the S phase,the number of invaded cells and the number of migrated cells were decreased,the differences were statistical significance(P<0.05).Compared with the miR-577 group and the miR-577+pcDNA group,HOXA1 mRNA and protein expression levels,proliferation activity of cells at 48 and 72 h,percentage of cells in S phase,number of invading cells and number of migrating cells in the miR-577+HOXA1 group were all increased,while percentage of cells in G0/G1 phase was decreased,the differences were statistical significance(P<0.05).Conclusion:miR-577 can inhibit proliferation,invasion and migration of MDB-MA-231 cells by targeting HOXA1.

关 键 词：miR-577 乳腺癌 HOXA1 增殖 侵袭 迁移 

分 类 号：R737.9[医药卫生—肿瘤]