微RNA-21与微RNA-19在老年分化型甲状腺癌患者中的表达及临床意义  

The expression and its clinical significance of microRNA-21 and microRNA-19 in differentiated thyroid carcinoma in elderly patients

作　　者：胡耀杰 罗晓燕[2] 刘倩 姜广建 陈春悠 Hu Yaojie;Luo Xiaoyan;Liu Qian;Jiang Guangjian;Chen Chunyou(Department of Head and Neck Surgery,Tangshan Gongren Hospital,Tangshan 063000,China;Department of Emergency,Kailuan General Hospital,Tangshan 063000,China;Diabetes Research Center,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]唐山工人医院头颈外科,063000 [2]开滦总医院急诊科,063000 [3]北京中医药大学糖尿病研究中心,100029

出　　处：《中华老年医学杂志》2021年第4期479-482,共4页Chinese Journal of Geriatrics

基　　金：唐山市甲状腺癌诊治基础创新团队资助(19130201C)。

摘　　要：目的:探讨微RNA-19(miR-19)和微RNA-21(miR-21)在老年分化型甲状腺癌(DTC)患者中的诊断价值,分析其与老年DTC患者病理特征的关系。方法:回顾性研究,入选2015年1月至2018年1月96例老年DTC患者,均行颈淋巴结穿刺和甲状腺癌根治手术,检测穿刺组织、DTC组织及癌旁组织的miR-21和miR-19表达水平,分析不同组织间miR-21和miR-19表达水平的相关性,观察不同临床病理特征的老年患者miR-21和miR-19表达水平的差异。结果:miR-21的表达水平由低到高分别为癌旁组织(0.92±0.33)、淋巴结组织(3.41±0.64)及DTC组织(4.28±1.56),差异有统计学意义(F=296.683,P<0.01);各组织中miR-19的表达水平差异无统计学意义(均P>0.05)。相关性检验结果显示,DTC组织miR-21表达水平与淋巴结组织、癌旁组织呈正相关(r值分别为0.724、0.801,均P<0.01)。DTC组织miR-19表达水平与淋巴结组织、癌旁组织无线性相关(r值分别为0.127、0.165,均P>0.05)。淋巴结组织miR-21的表达水平与癌旁组织呈正相关(r=0.705,P<0.01),而miR-19表达水平间无线性相关(r=0.191,P>0.05)。不同性别、不同肿瘤直径的患者颈淋巴结miR-21表达水平差异无统计学意义(P>0.05)。肿瘤分期Ⅲ期的患者颈淋巴结miR-21表达水平高于Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。存在腺体外浸润的患者颈淋巴结miR-21表达水平高于无腺外浸润的患者(P<0.05)。不同性别、肿瘤直径、肿瘤分期及肿瘤是否有腺外浸润的患者颈淋巴结miR-19表达水平差异无统计学意义(均P>0.05)。结论:miR-19可能不参与老年DTC的发生、发展,而miR-21在老年DTC的发生、发展中具有重要作用,尤其是在肿瘤的周围浸润与转移过程中可能存在有促进作用。颈淋巴结穿刺检测miR-21能够早期对DTC进行诊断与评估,为治疗方案的选择提供依据。Objective To investigate the diagnostic value of microRNA-19(miR-19)and microRNA-21(miR-21)in elderly patients with differentiated thyroid carcinoma(DTC),and to analyze the relationship of miR-19 and miR-21 expression with pathological characteristics in elderly patients with DTC.Methods A total of ninety-six elderly DTC patients visiting our hospital from January 2015 to January 2018 were included in this retrospective study.They underwent cervical lymph node puncture biopsy and radical surgery for thyroid cancer.The expression levels of miR-21 and miR-19 were detected in lymph node tissues,thyroid cancer tissues and normal para-cancerous tissue.The differences in miR-21 and miR-19 expression levels between three different pathological tissues in elderly patients were analyzed.The correlation of miR-21 and miR-19 expression levels with different clinico-pathological features in elderly patients was observed.Results The expression levels of miR-21 from low to high were(0.92±0.33)in para-cancerous tissue,(3.41±0.64)in lymph node tissue and(4.28±1.56)in DTC tissue respectively(F=296.683,P<0.01),the difference was statistically significant(F=296.683,P=0.01).While,there was no significant difference in the expression levels of miR-19 among different tissues(P>0.05).Spearman correlation test showed that the expression level miR-21 in DTC tissue was positively correlated with that in lymph node tissue and para-cancerous tissue(r values were 0.724,0.801,all P<0.01),while the expression level of miR-19 showed no correlation of DTC tissues with lymph node and para-cancerous tissues(r=0.127 and 0.165,P>0.05).The expression level of miR-21 in lymph node tissue was positively correlated with that in para-cancerous tissues(r=0.705,P<0.01),but the expression level of microRNA-19 had no correlation between the two kinds of tissues(r=0.191,P>0.05).There was no significant difference in the expression of miR-21 in cervical lymph nodes among patients with different gender and tumor diameter(P>0.05).The expression level of miR-2

关 键 词：甲状腺肿瘤 穿刺术 微RNAS 肿瘤转移 

分 类 号：R736.1[医药卫生—肿瘤]