基于VEGF信号通路研究苗药血脉通对脑出血大鼠脑组织细胞自噬与凋亡的作用  

Effects of Xuemaitong on autophagy and apoptosis in brain tissue of rats with intracerebral hemorrhage based on VEGF signaling pathway

作　　者：饶斌[1] 查昀 钟蕾[1] 姚益群[2] RAO Bin;ZHA Yun;ZHONG Lei;YAO Yiqun(Huangshi Fifth Hospital, Huangshi 420204, Hubei, China;Huangshi Central Hospital, Huangshi 435000, Hubei, China)

机构地区：[1]黄石市第五医院,湖北黄石420204 [2]黄石市中心医院,湖北黄石435000

出　　处：《西部医学》2021年第5期660-664,669,共6页Medical Journal of West China

基　　金：湖北省自然科学基金(2015CFB627)。

摘　　要：目的探讨基于血管内皮生长因子(VEGF)信号通路研究苗药血脉通对脑出血大鼠脑组织细胞自噬、凋亡的作用及其相关的作用机制。方法选取正常Wistar雄性大鼠50只,将12只大鼠作为假手术组,其余按照Rosenberg法建立脑出血大鼠模型随机分为脑出血组、给药A组和给药B组。给药A组和给药B组分别予以15 mg/(kg·d)、30 mg/(kg·d)苗药血脉通治疗,假手术组、脑出血组予以等量生理盐水,给药方式为灌胃,均连续给药35 d。采用TUNEL法检测各组大鼠脑组织细胞凋亡情况;采用Weatern blot法检测Beclin-1、VEGF、基质细胞衍生因子1(SCDF-1α)、CXCR-4蛋白表达量,免疫组化法检测PI3K、AKT蛋白水平;测定各组大鼠脑出血病灶体积。结果脑出血组、给药A组和给药B组大鼠脑组织细胞凋亡数、脑出血病灶体积、Beclin-1、VEGF、PI3K、AKT、SCDF-1α、CXCR-4等蛋白表达量均显著高于假手术组(P<0.05)。与脑出血组比较,给药A组和B组大鼠脑组织细胞凋亡数、脑出血病灶体积均显著降低(P<0.05),Beclin-1、VEGF、PI3K、AKT、SCDF-1α、CXCR-4等蛋白表达量均显著升高,差异具有统计学意义(P<0.05)。结论苗药血脉通治疗脑出血可通过激活VEGF信号通路调节细胞自噬,抑制细胞凋亡,发挥脑保护作用。Objective To study the effects of Miao medicine Xuemaitong on autophagy and apoptosis of brain tissue in rat models of cerebral hemorrhage based on VEGF signaling pathway and its corresponding role mechanism.Methods Rat models of cerebral hemorrhage were established according to the Rosenberg method.Rats were randomly divided into sham operation group,cerebral hemorrhage group,administration group A and administration group B.Administration group A and administration group B were treated with 15 mg/(kg·d)and 30 mg/(kg·d)of Miao medicine Xuemaitong respectively.The sham operation group and cerebral hemorrhage group were given the same amount of normal saline,and they were given for 35 d.The apoptosis of each group was detected by TUNEL method.The expression of Beclin-1,VEGF,stromal cell-derived factor 1(SCDF-1α)and CXCR-4 protein was detected by Western blot.The protein levels of PI3K andAKT were detected by immunohistochemistry.The volume of hemorrhagic lesions was measured in each group.Results The quantity of apoptotic cells and the volume of hemorrhagic lesions in cerebral hemorrhage group,administration group A and administration group B were significantly higher than those in sham operation group B,and the protein expression levels of Beclin-1,VEGF,PI3K,AKT,stromal cell-derived factor 1 and CXCR-4 were significantly lower than those in sham operation group.Compared with cerebral hemorrhage group,the quantity of apoptotic cells and the volume of hemorrhagic lesions in administration group A and administration group B were significantly decreased while the protein expression levels of Beclin-1,VEGF,PI3K,AKT,stromal cell-derived factor 1 and CXCR-4 were significantly increased(P<0.05).Conclusion Miao medicine Xuemaitong can treat brain cerebral hemorrhage by activating VEGF signaling pathway,regulating cell autophagy,inhibiting apoptosis and exerting brain protection.

关 键 词：VEGF信号通路 苗药血脉通 脑出血 自噬 凋亡 

分 类 号：R743.34[医药卫生—神经病学与精神病学]