c-Src与p-STAT3信号转导通路在上皮性卵巢癌发生、发展的关系研究  

The Relationship between c-Src and p-STAT3 Signaling Pathway in the Occurrence and Development of Epithelial Ovarian Cancer

作　　者：杨晓荣 谭娜 黄佳佳 刘慧之 王凯 郑洪 YANG Xiaorong;TAN Na;HUANG Jiajia(Department of Pathology,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563000,China)

机构地区：[1]遵义医科大学附属医院病理科,贵州遵义563000

出　　处：《实用妇产科杂志》2021年第4期291-296,共6页Journal of Practical Obstetrics and Gynecology

基　　金：贵州省科技厅基金(编号:[2015]7454号)。

摘　　要：目的:探讨原癌基因c-Src与磷酸化信号转导及转录活化因子3(p-STAT3)在上皮性卵巢癌(EOC)中的表达情况及临床意义。方法:应用实时荧光定量PCR及免疫组化SP法检测c-Src及p-STAT3蛋白分别在50例EOC癌组织(恶性组)、30例卵巢上皮性交界性肿瘤(交界性组)、30例卵巢上皮性良性肿瘤(良性组)及30例正常卵巢组织(正常组)中的表达情况,分析c-Src、p-STAT3与EOC临床病理参数的关系,及c-Src与p-STAT3在EOC癌组织中的相关性。结果:(1)恶性组中c-Src蛋白和p-STAT3蛋白的表达水平均高于交界性组、良性组及正常组,差异有统计学意义(P<0.05);(2)在恶性组中:c-Src蛋白阳性表达率(76.0%)高于交界性组(10.0%)、良性组(3.3%)及正常组(3.3%);p-STAT3蛋白阳性表达率(82.0%)高于交界性组(26.7%)、良性组(16.7%)及正常组(6.7%),差异均有统计学意义(P<0.05);(3)临床分期晚期(Ⅲ~Ⅳ期)患者的c-Src蛋白阳性表达率(92.6%)和p-STAT3蛋白阳性表达率(92.6%)均高于临床分期早期(Ⅰ~Ⅱ期)患者的c-Src蛋白阳性表达率(56.5%)和p-STAT3阳性表达率(69.6%);伴淋巴结转移患者的c-Src蛋白阳性表达率(100.0%)和p-STAT3蛋白阳性表达率(100.0%)高于无淋巴结转移患者的c-Src蛋白阳性表达率(72.1%)和p-STAT3蛋白阳性表达率(79.1%),差异均有统计学意义(P<0.05);(4)恶性组中c-Src蛋白的平均光密度值(MOD)与p-STAT3蛋白的MOD呈正相关(r=0.462,P<0.05)。结论:c-Src、p-STAT3参与了EOC的发生、发展并具有协同作用,联合检测c-Src、p-STAT3可为EOC早期诊断、鉴别诊断及分子靶向治疗提供理论依据。Objective:To investigate the expression and clinical significance of proto oncogene c-Src and p-STAT3 in epithelial ovarian cancer(EOC).Methods:The expression of c-Src and P-STAT3 protein in 50 cases of EOC cancer(malignant group),30 cases of epithelial borderline tumor(borderline group),30 cases of benign ovarian tumor(benign group) and 30 cases of normal ovarian tissue(normal group) were detected by real-time fluorescent quantitative PCR and immunohistochemistry SP method.The relationship between c-Src, p-STAT3 and clinicopathological parameters of EOC,and the correlation between c-Src and p-STAT3 in EOC carcinoma were analyzed.Results:(1)The expression levels of c-Src and p-STAT3 in malignant group were significantly higher than those in borderline group, benign group and normal group(P<0.05);(2)The positive rate of c-Src expression in malignant group(76.0%) was significantly higher than that in borderline group(10.0%),benign group(3.3%) and normal group(3.3%);the positive rate of p-STAT3 expression in malignant group(82.0%) was significantly higher than that in borderline group(26.7%),benign group(16.7%) and normal group(6.7%)(P<0.05);(3)The positive rates of c-Src and p-STAT3 in patients with advanced clinical stage(stage Ⅲ-Ⅳ) were 92.6% and 92.6%,respectively, which were higher than those in patients with early clinical stage(stage Ⅰ-Ⅱ)(56.5%) and p-STAT3(69.6%);the positive rates of c-Src protein expression and p-STAT3 protein expression in patients with lymph node metastasis(100.0%,100.0%) were significantly higher than those in patients without lymph node metastasis(72.1%,79.1%,respectively)(P<0.05);(4)The mean optical density(MOD) of c-Src protein was positively correlated with that of p-STAT3 protein in malignant group(r=0.462,P<0.05).Conclusions:The c-Src and p-STAT3 could participate in the occurrence and development of EOC with synergistic effect.The joint detection of c-Src and p-STAT3 can provide theoretical basis for early diagnosis, differential diagnosis and molecular targeted therapy o

关 键 词：上皮性卵巢癌 C-SRC P-STAT3 实时荧光定量PCR 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]