原发性胆汁性胆管炎中IgG亚型分布特征分析  

Distribution of IgG subtypes in primary biliary cholangitis

作　　者：郑微 姜菲菲[1] 单晶[2] 王颖[1] 贾咏梅 郭秋艳[1] 娄金丽[1] 赵艳[1] Zheng Wei;Jiang Feifei;Shan Jing;Wang Ying;Jia Yongmei;Guo Qiuyan;Lou Jinli;Zhao Yan(Clinical Laboratory Center,Beijing Youan Hospital,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院临检中心,100069 [2]首都医科大学附属北京佑安医院肝病免疫科,100069

出　　处：《北京医学》2021年第3期207-210,共4页Beijing Medical Journal

基　　金：北京市自然科学基金(7191004,7202069);北京市科技计划(Z171100001017078);北京市重点实验室项目(BZ0373);首都卫生发展科研专项(首发2020-2-1153);北京市医院管理局“登峰”人才培养计划(DFL20181701);北京市医院管理局重点医学专业发展计划(ZYLX201711)。

摘　　要：目的探讨原发性胆汁性胆管炎(primary biliary cholangitis,PBC)患者血清中免疫球蛋白G(immunoglobulin G,IgG)亚型的分布特征。方法选取2018年5月至2019年3月首都医科大学附属北京佑安医院收治的28例PBC患者、29例自身免疫性肝炎(autoimmune hepatitis,AIH)患者及同期30名健康体检者。散射比浊法检测血清IgG1、IgG2、IgG3、IgG4水平;分析IgG亚型水平及IgG亚型占总IgG水平百分比的分布特征。结果IgG1、IgG1/IgG、IgG3、IgG3/IgG在3组中的分布差异均有统计学意义(P<0.05),其中AIH组以IgG1、IgG1/IgG升高为特点,PBC组以IgG3、IgG3/IgG升高为特点。PBC组的IgG3与IgM水平呈显著正相关(r=0.709,P<0.001)。PBC患者中IgG3水平正常组与IgG3升高组抗线粒体抗体(AMA)的分布差异有统计学意义(χ^(2)=6.910,P=0.030),IgG3升高组的AMA-M2显著高于IgG3正常组[662.4(418.8,794.4)RU/ml比199.5(25.0,308.9)RU/ml,Z=30.000,P=0.003]。结论PBC患者的IgG水平显著升高,且IgG抗体亚型以IgG3升高为主,IgG3升高可能与高滴度AMA和AMA-M2抗体有关。Objective To investigate the distribution of immunoglobulin G(IgG)subtypes in the patients with primary biliary cholangitis(PBC).Methods Twenty-eight PBC patients,29 autoimmune hepatitis(AIH)patients and 30 healthy controls(HCs)were collected from May 2018 to March 2019 in Beijing Youan Hospital.The levels of IgG1,IgG2,IgG3 and IgG4 in serum were detected by nephelometry.The levels and distribution characteristics of IgG subtype were analyzed.Results The distribution of IgG1,IgG1/IgG,IgG3,and IgG3/IgG in the three groups was statistically significant(P<0.05).IgG1 and IgG1/IgG were elevated in the AIH group,IgG3 and IgG3/IgG were elevated in the PBC group.There was a significant positive correlation between IgG3 and IgM in the PBC group(r=0.709,P<0.001).The distribution of AMA between the normal IgG3 group and the elevated IgG3 group was statistically significant(χ^(2)=6.910,P=0.030).AMA-M2 in the elevated IgG3 group was significantly higher than that in the normal IgG3 group[662.4(418.8,794.4)RU/ml vs.199.5(25.0,308.9)RU/ml,Z=30.000,P=0.003].Conclusions The level of IgG is significantly increased in the PBC patients,and the level of IgG3 is mainly increased.The increase of IgG3 may be related to high AMA and AMA-M2.

关 键 词：原发性胆汁性胆管炎 免疫球蛋白G 亚型 

分 类 号：R575.7[医药卫生—消化系统]