基于气相色谱法测定食品中甜蜜素的碱性水解法研究  

Alkaline Hydrolysis Method for Determination of Sodium Cyclamate in Food Using Gas Chromatography

作　　者：刘志鹏 杨李胜 王小鹏 黄敏兴 林继钦 余构彬 陈其钊 LIU Zhi-peng;YANG Li-sheng;WANG Xiao-peng;HUANG Min-xing;LIN Ji-qin;YU Gou-bin;CHEN Qi-zhao(Institute of Bioengineering,Guangdong Academy of Sciences,Guangzhou 510316,Guangdong,China;Research Center for Sugarcane Industry Engineering Technology of Light Industry of China,Guangzhou 510316,Guangdong,China)

机构地区：[1]广东省科学院生物工程研究所,广东广州510316 [2]中国轻工业甘蔗制糖工程技术研究中心,广东广州510316

出　　处：《食品研究与开发》2021年第10期154-158,共5页Food Research and Development

基　　金：广东省科学院发展专项资金项目(2019GDASYL-0105037);广州市科技计划项目(201806010093)。

摘　　要：对国标GB 5009.97—2016《食品安全国家标准食品中环己基氨基磺酸钠的测定》进行方法改进,前处理步骤为:称取样品,定容,加入正庚烷后立即添加亚硝酸钠和硫酸反应,冰浴后水解,最后加入氯化钠振摇,取上清液分析,通过测定正庚烷提取液的环己醇含量,间接准确测定甜蜜素含量。该方法与国标相比具有重现性好、准确度高、精密度高的优点,其中方法检出限为0.008 g/kg,相关系数R2为0.9996,回收率在94.0%~104.0%之间,相对标准偏差在3.02%~4.75%之间。This study aimed to improve the national standard method GB 5009.97—2016 National Food Safety Standards:Determination of Sodium Cyclamate in Food.During the process of sample pretreatment,n-heptane was added after weighing and measuring the constant volume of the samples.Then,sodium nitrite and vitriol were immediately mixed with the samples on the ice.Finally,sodium chloride was added.The cyclohexanol in the supernatant of the n-heptane extracting solution,which was representative of sodium cyclamate,was detected using gas chromatography.The detection limit was 0.008 g/kg,the correlation coefficient of the calibration curves was 0.9996,and the average recovery rates were in the range of 94.0%-104.0%.Moreover,the relative standard deviations were in the range of 3.02%-4.75%.

关 键 词：甜蜜素 气相色谱 碱性水解 外标法 环己醇 

分 类 号：O657.71[理学—分析化学] TS207.3[理学—化学]