远海50个站位沉积物中潜在木质素降解菌的分离与鉴定  

Isolation and identification of potential lignin-degrading bacteria in sediments from 50 stations of the Pelagic Ocean

作　　者：黄一哲 任娜 徐莹 吴李园 董冰夏 朱四东 杨季芳[1] 陈吉刚[1] HUANG Yizhe;REN Na;XU Ying;WU Liyuan;DONG Bingxia;ZHU Sidong;YANG Jifang;CHEN Jigang(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo,Zhejiang 315100,China)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《微生物学通报》2021年第4期1080-1092,共13页Microbiology China

基　　金：宁波市公益类科技计划(202002N3115);全球变化与海气相互作用专项(二期)(GASI-04-HYDZ-02);中国大洋矿产资源研究开发协会项目(DY135-E2-2-07);浙江省生物工程一流学科创新基金(CX2019004)。

摘　　要：【背景】微生物在海洋木质素的降解过程中发挥了至关重要的作用,然而来源海洋环境的木质素降解菌的相关研究报道却很少。【目的】从远海沉积物环境分离潜在的木质素降解菌,为木质素的可再生化学物质转化提供菌种资源。【方法】利用以木质素为唯一碳源的培养基,对50个远海沉积物样品中的木质素降解菌进行富集培养与纯化,并利用含苯胺蓝的脱色培养基筛选潜在木质素降解菌,继而通过16S rRNA基因测序与序列比对初步确定潜在木质素降解菌的分类地位。【结果】从50个沉积物样品中共分离获得菌株283株,其中潜在木质素降解菌263株,它们隶属于α-变形杆菌纲(Alphapreobacteria)、γ-变形杆菌纲(Gamaproteobacteria)、芽孢杆菌纲(Bacilli)、放线杆菌纲(Actinobacteria)和黄杆菌纲(Flavobacteriia)中的32个属。【结论】远海沉积物环境中蕴含着丰富的木质素降解菌,为海洋生物资源的开发利用提供了新的参考。[Background] Microorganisms play a vital role in the degradation process of marine lignin, but there are few reports about the lignin-degrading bacteria that originate from the marine environment. [Objective] To isolate potential lignin-degrading bacteria from sediments of Pelagic Ocean, and to provide strain resources for the conversion of renewable chemical substances of lignin. [Methods] Enrichment and purification of lignin-degrading bacteria in 50 sediment samples from the Pelagic Ocean by using the medium with alkaline lignin as the sole carbon source, and then screen for potential lignin-degrading bacteria by using the decolorizing medium. The taxonomic status of potential lignin-degrading bacteria preliminarily determined by 16S rRNA gene sequencing and sequence identity comparison. [Results] A total of 283 strains were isolated from 50 sediment samples, including 263 potential lignin-degrading strains, which belonged to 32 genera in five class(Alphapreobacteria, Gamaproteobacteria, Bacilli, Actinobacteria and Flavobacteriia). [Conclusion] The sediment environment in the Pelagic Ocean is rich in lignin-degrading bacteria, which provided new references for the development and utilization of marine biological resources.

关 键 词：远海 木质素降解菌 鉴定 

分 类 号：X172[环境科学与工程—环境科学]