基于双二茂铁和β-环糊精之间主客体识别模式信号放大的均相电化学适体传感器用于凝血酶的灵敏检测  

A homogeneous electrochemical aptasensor based on signal amplification mode of host-guest recognition between bisferrocene andβ-CD for sensitive detection of thrombin

作　　者：黄靓[1,2] 徐慧慧 熊魏 马广强[2] 廖夫生 王梅[2] 崔汉峰 HUANG Liang;XU Huihui;WEI Xiong;MA Guangqiang;LIAO Fusheng;WANG Mei;CUI Hanfeng(Nanchang No.3 Middle School,Nanchang 330000;Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004)

机构地区：[1]南昌市第三中学,南昌330000 [2]江西中医药大学药学院,南昌330004

出　　处：《分析试验室》2021年第4期380-384,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81560625)资助。

摘　　要：合成了双二茂铁化合物并用于修饰在凝血酶适配体(TBA)的两端作为电化学信号标记物,构建了一款基于双二茂铁与β-环糊精(β-CD)之间主客体识别原理进行信号扩增的均相电化学凝血酶传感器。当电化学TBA探针与凝血酶发生特异性结合后,TBA探针由原来的茎环结构变成"G-四链体",双二茂铁分子通过主客体识别作用进入修饰在金电极表面的β-CD的空腔内,产生了稳定的电化学电流响应信号。该凝血酶电化学均相传感器在0.02~62.5 nmol/L范围内对凝血酶呈良好的线性关系,检出限为8.4 pmol/L。该传感器对凝血酶可为凝血酶的快速检测提供了一个可行的方案。In this study,bisferrocene was synthesized,and modified on both terminals of thrombin aptamer(TBA)as electrochemical signal marker.Subsequently,an electrochemical homogeneous aptasensor for the sensitive and rapid detection of thrombin was constructed by a signal amplification mode of host-guest recognition between bisferrocene andβ-cyclodextrin(β-CD).After binding of thrombin,TBA changed from the original stem-loop structure to"G-quadruplex",and the bisferrocene entered the cavity ofβ-CD modified on the surface of the gold electrode through the host-guest recognition,generating a stable electrochemical current signal.This signal amplification strategy revealed a dynamic range from 20 pmol/L to 62.5 nmol/L with a detection limit of8.4 pmol/L.This aptasensor shows high specificity and satisfactory stability for thrombin,and provides a feasible solution for the rapid detection of thrombin.

关 键 词：双二茂铁 凝血酶 Β-环糊精 主客体识别作用 电化学均相传感器 

分 类 号：O657.1[理学—分析化学]