自然杀伤细胞、白细胞介素27及CXC趋化因子配体10在三阴性乳腺癌中的表达及其临床意义  

Expressions of natural killer cells,interleukin-27 and CXC chemokine ligand 10 in triple-negative breast cancer and their clinical significances

作　　者：马苗苗 张泽高[1] 普拉提·努尔别克[1] 杨杰[1] Ma Miaomiao;Zhang Zegao;Pulat·Nurbek;Yang Jie(Department of Radiotherapy 2,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830011,China)

机构地区：[1]新疆维吾尔自治区人民医院放疗二科,乌鲁木齐830011

出　　处：《肿瘤研究与临床》2021年第3期189-194,共6页Cancer Research and Clinic

基　　金：新疆维吾尔自治区青年基金(2017D01C148)。

摘　　要：目的探讨自然杀伤(NK)细胞、白细胞介素27(IL-27)和CXC趋化因子配体10(CXCL10)在三阴性乳腺癌和非三阴性乳腺癌患者中的表达及其临床意义。方法选取2012年1月至2016年6月新疆维吾尔自治区人民医院经病理确诊的三阴性乳腺癌48例、非三阴性乳腺癌50例及乳腺良性疾病50例。采用免疫组织化学法检测患者术后组织石蜡切片中NK细胞、IL-27和CXCL10表达情况,并分析其与患者临床特征及预后的关系。结果NK细胞在三阴性乳腺癌、非三阴性乳腺癌、乳腺良性疾病组织中的阳性表达率分别为81.25%(39/48)、64.00%(32/50)、26.00%(13/50),差异有统计学意义(χ^(2)=28.229,P<0.01);IL-27在三组中的阳性表达率分别为85.42%(41/48)、76.00%(35/50)、16.00%(10/50),差异有统计学意义(χ^(2)=47.439,P<0.01);CXCL10在三组中的阳性表达率分别为72.92%(35/48)、62.00%(31/50)、18.00%(9/50),差异有统计学意义(χ^(2)=33.422,P<0.01)。三阴性乳腺癌中,有淋巴结转移者NK细胞阳性率高(χ^(2)=7.134,P=0.008),肿瘤长径>2 cm者IL-27阳性率高(P=0.034),肿瘤分期晚、有淋巴结转移者CXCL10阳性率高(χ^(2)=8.574,P=0.003;χ^(2)=4.789,P=0.049);非三阴性乳腺癌中,肿瘤长径>2 cm者IL-27阳性率高(χ^(2)=6.711,P=0.010),肿瘤组织学分级高者CXCL10阳性率高(χ^(2)=7.621,P=0.006)。NK细胞阳性与阴性的三阴性乳腺癌患者间总生存差异有统计学意义(P=0.047),NK细胞阳性患者预后更差。结论NK细胞、IL-27及CXCL10表达与乳腺癌的发生、发展有关,NK细胞可作为三阴性乳腺癌患者的潜在预后评估指标和临床治疗的靶点。Objective To investigate the expressions of natural killer(NK)cells,interleukin-27(IL-27)and CXC chemokine ligand 10(CXCL10)in triple-negative breast cancer patients and non-triple-negative breast cancer patients and their clinical significances.Methods Forty-eight cases of triple-negative breast cancer,50 cases of non-triple-negative breast cancer and 50 cases of benign breast diseases diagnosed by pathology in the People's Hospital of Xinjiang Uygur Autonomous Region from January 2012 to June 2016 were selected.The expression of NK cells,IL-27 and CXCL10 in the paraffin sections of tissues of the patients after surgery were detected by immunohistochemistry,and the relationships between them and clinical features and prognosis of the patients were analyzed.Results The positive expression rates of NK cells in triple-negative breast cancer,non-triple-negative breast cancer and benign breast diseases tissues were 81.25%(39/48),64.00%(32/50)and 26.00%(13/50),respectively,and the difference was statistically significant(χ^(2)=28.229,P<0.01);the positive expression rates of IL-27 in the three groups were 85.42%(41/48),76.00%(35/50)and 16.00%(10/50),and the difference was statistically significant(χ^(2)=47.439,P<0.01);the positive expression rates of CXCL10 in the three groups were 72.92%(35/48),62.00%(31/50)and 18.00%(9/50),and the difference was statistically significant(χ^(2)=33.422,P<0.01).In the triple-negative breast cancer group,the positive rate of NK cells was higher in patients with lymph node metastasis(χ^(2)=7.134,P=0.008),the positive rate of IL-27 was higher in patients with tumor long-axis diameter>2 cm(P=0.034),and the positive rate of CXCL10 was higher in patients with late tumor stage and lymph node metastasis(χ^(2)=8.574,P=0.003;χ^(2)=4.789,P=0.049).In the non-triple-negative breast cancer group,the positive rate of IL-27 was higher in patients with tumor long-axis diameter>2 cm(χ^(2)=6.711,P=0.010),and the positive rate of CXCL10 was higher in patients with high tumor histological grade(χ^(2

关 键 词：乳腺肿瘤 三阴性乳腺癌 非三阴性乳腺癌 免疫组织化学 杀伤细胞 天然 白细胞介素27 趋化因子CXCL10 

分 类 号：R737.9[医药卫生—肿瘤]